Water-soluble poly(acrylamide-allylamine) derivatives of saccharides for protein-saccharide binding studies
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
7795412
DOI
10.1007/bf00731868
Knihovny.cz E-zdroje
- MeSH
- akrylové pryskyřice chemie MeSH
- allylamin chemie MeSH
- lektiny chemie metabolismus MeSH
- ligandy MeSH
- polymery chemie MeSH
- polysacharidy chemie metabolismus MeSH
- proteiny chemie metabolismus MeSH
- rozpustnost MeSH
- voda chemie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- akrylové pryskyřice MeSH
- allylamin MeSH
- lektiny MeSH
- ligandy MeSH
- polyacrylamide MeSH Prohlížeč
- polymery MeSH
- polysacharidy MeSH
- proteiny MeSH
- voda MeSH
Water-soluble poly(acrylamide-allylamine) copolymers containing covalently bound amino groups, prepared by copolymerization of acrylamide and allylamine, can be used as general carriers for coupling of different types of saccharides or saccharide derivatives. The water-soluble macromolecular carbohydrate derivatives can be easily labelled and used in various solid-phase techniques to study protein-saccharide interaction. Two types of coupling reaction were used to prepare polyacrylamide derivatives of saccharides: reductive amination was applied to couple the reducing disaccharides and a carbodiimide reaction was used to couple heparin via its carboxyl groups to the amino groups of the poly(acrylamide-allylamine) derivative. Peroxidase labelled or biotinylated derivatives were shown to be useful in studies on the binding properties of lectins and proteins from boar seminal plasma.
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