Biochemical, morphological and cytochemical studies of enhanced autolysis of Saccharomyces cerevisiae. 2. Morphological and cytochemical studies
Language English Country United States Media print
Document type Journal Article
PubMed
8150396
DOI
10.1007/bf02814399
Knihovny.cz E-resources
- MeSH
- Autolysis enzymology pathology MeSH
- Sodium Chloride pharmacology MeSH
- Microscopy, Electron MeSH
- Endopeptidases metabolism MeSH
- Ethanol pharmacology MeSH
- Histocytochemistry MeSH
- Intracellular Membranes enzymology MeSH
- Carboxypeptidases metabolism MeSH
- Cathepsin A MeSH
- Microscopy, Electron, Scanning MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Saccharomyces cerevisiae drug effects enzymology ultrastructure MeSH
- Vacuoles enzymology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Sodium Chloride MeSH
- Endopeptidases MeSH
- Ethanol MeSH
- Carboxypeptidases MeSH
- Cathepsin A MeSH
- PRC1 protein, S cerevisiae MeSH Browser
- Saccharomyces cerevisiae Proteins MeSH
- serine carboxypeptidase MeSH Browser
Morphological and cytochemical observation of Saccharomyces cerevisiae undergoing of induced autolysis were done in response to various chemical inducers of autolysis (NaCl, ethanol, fresh autolyzate). Changes in the inner structure of yeast cells were monitored by transmission electron microscopy and the surface of the cell wall was observed by scanning electron microscopy during autolysis. Cytochemical characterization of autolyzed cells was performed using four synthetic substrates for determination of proteinase activities but only carboxypeptidase Y could be detected in the vacuolar membranes. The morphological studies supported the data obtained from biochemical studies and confirmed that optimized conditions of autolysis have a significant effect on the structural changes of autolyzed yeast.
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