Biochemical, morphological and cytochemical studies of enhanced autolysis of Saccharomyces cerevisiae. 2. Morphological and cytochemical studies
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
8150396
DOI
10.1007/bf02814399
Knihovny.cz E-zdroje
- MeSH
- autolýza enzymologie patologie MeSH
- chlorid sodný farmakologie MeSH
- elektronová mikroskopie MeSH
- endopeptidasy metabolismus MeSH
- ethanol farmakologie MeSH
- histocytochemie MeSH
- intracelulární membrány enzymologie MeSH
- karboxypeptidasy metabolismus MeSH
- kathepsin A MeSH
- mikroskopie elektronová rastrovací MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- Saccharomyces cerevisiae účinky léků enzymologie ultrastruktura MeSH
- vakuoly enzymologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorid sodný MeSH
- endopeptidasy MeSH
- ethanol MeSH
- karboxypeptidasy MeSH
- kathepsin A MeSH
- PRC1 protein, S cerevisiae MeSH Prohlížeč
- Saccharomyces cerevisiae - proteiny MeSH
- serine carboxypeptidase MeSH Prohlížeč
Morphological and cytochemical observation of Saccharomyces cerevisiae undergoing of induced autolysis were done in response to various chemical inducers of autolysis (NaCl, ethanol, fresh autolyzate). Changes in the inner structure of yeast cells were monitored by transmission electron microscopy and the surface of the cell wall was observed by scanning electron microscopy during autolysis. Cytochemical characterization of autolyzed cells was performed using four synthetic substrates for determination of proteinase activities but only carboxypeptidase Y could be detected in the vacuolar membranes. The morphological studies supported the data obtained from biochemical studies and confirmed that optimized conditions of autolysis have a significant effect on the structural changes of autolyzed yeast.
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