The behavior of cells during the evaluation of gap junctional intercellular communication by metabolic cooperation assay
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8843974
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- Cricetulus MeSH
- dibutyryl cyklický AMP farmakologie MeSH
- ethylenglykol MeSH
- ethylenglykoly farmakologie MeSH
- fibroblasty cytologie účinky léků metabolismus MeSH
- křečci praví MeSH
- mezerový spoj účinky léků fyziologie MeSH
- mezibuněčná komunikace účinky léků fyziologie MeSH
- mitóza MeSH
- protinádorové antimetabolity farmakologie MeSH
- thioguanin farmakologie MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dibutyryl cyklický AMP MeSH
- ethylenglykol MeSH
- ethylenglykoly MeSH
- protinádorové antimetabolity MeSH
- thioguanin MeSH
The behavior of cells during the assessment of gap junctional intercellular communication (GJIC) by metabolic cooperation assay was studied, either under standard conditions, or with the simultaneous addition of an inhibitor of GJIC-ethylene glycol, and eventually plus dibutyryl cyclic adenosine monophosphate. Generally, the behavior of cells in the course of the assay did not depend significantly on the combination of drugs used. Cell locomotion during the assay was minimal, and consequently the results depended, almost solely on the probability of mutual contact between donor and recipient cells during the seeding. The first significant decrease in recovery of recipient cells, that is a detectable GJIC, was observed 30 min after the assay had started. Final results of the assay depended on the first 12 h of the experiment, in which the maximum decrease in the recovery of recipient cells, that is the maximum GJIC, was reached. The longer duration of the assay did not affect the results significantly.