Linkup of a fast protein liquid chromatography system with a stirred thermostated cell for sterile preparation of liposomes by the proliposome-liposome method: application to encapsulation of antibiotics, synthetic peptide immunomodulators, and a photosensitizer
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9212864
DOI
10.1006/abio.1997.2146
PII: S0003269797921461
Knihovny.cz E-resources
- MeSH
- Adjuvants, Immunologic administration & dosage chemical synthesis MeSH
- Anti-Bacterial Agents administration & dosage MeSH
- Photosensitizing Agents administration & dosage MeSH
- Drug Contamination MeSH
- Liposomes chemical synthesis MeSH
- Drug Carriers chemical synthesis MeSH
- Peptides administration & dosage chemical synthesis MeSH
- Temperature * MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Freezing MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Anti-Bacterial Agents MeSH
- Photosensitizing Agents MeSH
- Liposomes MeSH
- Drug Carriers MeSH
- Peptides MeSH
The proliposome-liposome method is based on the conversion of the initial proliposome preparation into a liposome dispersion by dilution with the aqueous phase. This technique is characterized by an extremely high entrapment efficiency and is suitable for the encapsulation of a wide range of drugs with different water and alcohol solubility. A description of a home-made stirred thermostated cell and its linkup with an FPLC system for a rapid and automated preparation of multilamellar liposomes under strictly controlled conditions (temperature, dilution rate, and schedule) is presented. The highly reproducible procedure yields multilamellar liposomes with a high encapsulation efficiency for various drugs. Carboxyfluorescein, as a model hydrophilic compound, was entrapped with an efficiency of 81 +/- 2%. The antibiotics neomycin and gentamycin were entrapped with efficiencies of 65 and 69%, respectively. Synthetic immunomodulators adamantylamide dipeptide, muramyl dipeptide, and beta-D-GlcNAc-norMurNAc-L-Abu-D-isoGln were entrapped with efficiencies of 87, 62, and 85%, respectively. The photosensitizer mesotetra-(parasulfophenyl)-porphin was entrapped with an efficiency of 65%. The cell has been designed for laboratory-scale preparation of liposomes (300-1000 mg of phospholipid per run) in a procedure taking less than 90 min. The method can be readily scaled up and linked with secondary processing methods, such as pressure extrusion through polycarbonate filters.
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