7Beta-OH-DHEA counteracts dexamethasone induced suppression of primary immune response in murine spleenocytes
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10659701
DOI
10.1016/s0960-0760(99)00134-x
PII: S0960-0760(99)00134-X
Knihovny.cz E-resources
- MeSH
- Antibody-Producing Cells cytology drug effects immunology MeSH
- Dehydroepiandrosterone administration & dosage analogs & derivatives pharmacology MeSH
- Dexamethasone antagonists & inhibitors pharmacology MeSH
- Hemolytic Plaque Technique MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Spleen cytology drug effects immunology MeSH
- In Vitro Techniques MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 7-hydroxydehydroepiandrosterone MeSH Browser
- Dehydroepiandrosterone MeSH
- Dexamethasone MeSH
The effect of dexamethasone and of three potential antiglucocorticoids, namely dehydroepiandrosterone (DHEA) and its 7alpha-and 7beta-hydroxylated metabolites, on primary immune response has been studied by measuring the number of plaque forming cells (NPFC) and their viability in a cell culture of murine spleenocytes. As expected, dexamethasone suppressed considerably the NPFC as well as their viability. Surprisingly, DHEA as well as its 7alpha-hydroxylated metabolite decreased significantly the NPFC, while the effect of 7beta-hydroxy-DHEA was different: at low doses it decreased the NPFC, but this effect was less pronounced at higher concentrations. In addition, 7beta-hydroxy-DHEA was able to counteract the effect of dexamethasone on the NPFC. None of the natural steroids affected the cell viability.
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