Use of synchronously excited fluorescence to assess the accumulation of membrane potential probes in yeast cells
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11271805
DOI
10.1007/bf02908949
Knihovny.cz E-resources
- MeSH
- Fluorescent Dyes metabolism MeSH
- Spectrometry, Fluorescence methods MeSH
- Carbocyanines metabolism MeSH
- Membrane Potentials physiology MeSH
- Saccharomyces cerevisiae physiology MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3,3'-dipropylthiacarbocyanine MeSH Browser
- Fluorescent Dyes MeSH
- Carbocyanines MeSH
Evaluation of emission spectra of fluorescent probes used for the monitoring of membrane potential in microbial cells can be greatly facilitated by using synchronously excited spectroscopy (SES). This method permits the suppression of undesirable spectrum components (contributions due to scattered light or cell autofluorescence) and leads to considerable increase in monitored emission intensity and to narrowing of spectral peaks. It allows an efficient fractional decomposition of the probe fluorescence spectra into their free and bound dye fluorescence components. The usefulness of the method was tested by monitoring the accumulation of the fluorescent membrane potential probe diS-C3(3) in yeast cells, which serves as a qualitative measure of the membrane potential.
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