Metabolism of branched-chain amino acids in starved rats: the role of hepatic tissue
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11300224
Knihovny.cz E-zdroje
- MeSH
- alanintransaminasa analýza MeSH
- aspartátaminotransferasy analýza MeSH
- draslík analýza MeSH
- glukosa analýza MeSH
- hladovění metabolismus patologie MeSH
- isoleucin metabolismus MeSH
- játra metabolismus patologie MeSH
- ketokyseliny metabolismus MeSH
- kosterní svaly metabolismus MeSH
- krysa rodu Rattus MeSH
- leucin metabolismus MeSH
- oxidace-redukce MeSH
- potkani Wistar MeSH
- proteiny metabolismus MeSH
- proteosyntéza MeSH
- sodík analýza MeSH
- valin metabolismus MeSH
- velikost orgánu MeSH
- větvené aminokyseliny krev metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alanintransaminasa MeSH
- alpha-ketoisocaproic acid MeSH Prohlížeč
- aspartátaminotransferasy MeSH
- draslík MeSH
- glukosa MeSH
- isoleucin MeSH
- ketokyseliny MeSH
- leucin MeSH
- proteiny MeSH
- sodík MeSH
- valin MeSH
- větvené aminokyseliny MeSH
Parameters of branched-chain amino acids (BCAA; leucine, isoleucine and valine) and protein metabolism were evaluated using L-[1-(14)C]leucine and alpha-keto[1-(14)C]isocaproate (KIC) in the whole body and in isolated perfused liver (IPL) of rats fed ad libitum or starved for 3 days. Starvation caused a significant increase in plasma BCAA levels and a decrease in leucine appearance from proteolysis, leucine incorporation into body proteins, leucine oxidation, leucine-oxidized fraction, and leucine clearance. Protein synthesis decreased significantly in skeletal muscle and the liver. There were no significant differences in leucine and KIC oxidation by IPL. In starved animals, a significant increase in net release of BCAA and tyrosine by IPL was observed, while the effect on other amino acids was non-significant. We conclude that the protein-sparing phase of uncomplicated starvation is associated with decreased whole-body proteolysis, protein synthesis, branched-chain amino acid (BCAA) oxidation, and BCAA clearance. The increase in plasma BCAA levels in starved animals results in part from decreased BCAA catabolism, particularly in heart and skeletal muscles, and from a net release of BCAA by the hepatic tissue.
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