Serum phospholipid fatty acid composition and insulin action in type 2 diabetic patients
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11735096
DOI
10.1053/meta.2001.27195
PII: S0026049501687341
Knihovny.cz E-resources
- MeSH
- C-Peptide blood MeSH
- Diabetes Mellitus, Type 2 blood MeSH
- Adult MeSH
- Phospholipids blood MeSH
- Glucose Clamp Technique MeSH
- Glycated Hemoglobin analysis MeSH
- Insulin blood pharmacology MeSH
- Insulin Resistance * MeSH
- Kinetics MeSH
- Blood Glucose metabolism MeSH
- Arachidonic Acid blood MeSH
- Linoleic Acid blood MeSH
- Fatty Acids, Omega-6 MeSH
- Middle Aged MeSH
- Humans MeSH
- Fatty Acids blood MeSH
- Metabolic Clearance Rate MeSH
- Fatty Acids, Unsaturated blood MeSH
- Fatty Acids, Omega-3 blood MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- C-Peptide MeSH
- Phospholipids MeSH
- Glycated Hemoglobin A MeSH
- Insulin MeSH
- Blood Glucose MeSH
- Arachidonic Acid MeSH
- Linoleic Acid MeSH
- Fatty Acids, Omega-6 MeSH
- Fatty Acids MeSH
- Fatty Acids, Unsaturated MeSH
- Fatty Acids, Omega-3 MeSH
Relationships have been demonstrated between insulin sensitivity and the fatty acid (FA) composition of serum and tissue lipids in adult humans. The present study aimed to investigate the above relationships in different groups of type 2 diabetic patients (DM2). The FA composition of serum phospholipids (S-PL) measured by gas liquid chromatography and insulin action during a 2-step hyperinsulinemic isoglycemic clamp (1 and 10 mU/kg. min) were determined in 21 newly diagnosed DM2 subjects (DMN), in groups of long-term DM2 patients treated with hypoglycemic agents (DMH; n = 21) or diet alone (DMD; n = 11), and in 24 healthy subjects (HS). In diabetics, the metabolic clearance rates of glucose at both insulin levels (MCR(glu)submax and MCR(glu)max) were significantly reduced compared with HS (MCR(glu)submax DMN, 5.35 +/- 2.7 mL x kg(-1) x min(-1), DMH, 5.38 +/- 2.17 mL x kg(-1) x min(-1); DMD, 5.48 +/- 2.35 mL x kg(-1) x min(-1) v HS, 10.9 +/- 3.3 mL x kg(-1) x min(-1); P <.01; MCR(glu)max DMN, 13.3 +/- 3.3 mL x kg(-1) x min(-1); DMH, 12.5 +/- 3.0 mL x kg(-1) x min(-1); DMD, 13.3 +/- 3.0 mL x kg(-1) x min(-1) v HS, 17.4 +/- 3.8 mL x kg(-1) x min(-1); P <.05). Increased contents of highly unsaturated n-6 family FA (P <.01), arachidonic acid in particular (DMN, 10.98% +/- 1.79%; DMD, 10.78% +/- 1.64%; DMH, 10.97% +/- 1.7% v HS, 8.51% +/- 1.53%; P <.001), were found in all groups of diabetics compared with HS, while lower levels of linoleic acid were seen in DMN (P <.001) and DMH (P <.05). The contents of saturated FA and monounsaturated FA were comparable in HS, DMN, and DMD. While in HS there were significant negative correlations between MCR(glu) and the contents of saturated FA and a positive association between insulin action and proportions of linoleic and arachidonic acids, no significant relationships were found in diabetic subjects. Different groups of DM2 patients show an altered FA pattern of S-PL, which is not related to insulin action. The above data support the hypothesis that changes in FA composition may play a role in modulating insulin action in peripheral tissues, but cannot explain the insulin resistance (IR) in DM2 patients.
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