Monitoring of in vitro deamidation of gliadin peptic fragment by mass spectrometry may reflect one of the molecular mechanisms taking place in celiac disease development
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12112756
DOI
10.1002/jms.305
Knihovny.cz E-zdroje
- MeSH
- aktivace makrofágů účinky léků MeSH
- celiakie metabolismus MeSH
- gliadin analýza farmakologie MeSH
- glutamin metabolismus MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací * MeSH
- interleukin-10 metabolismus MeSH
- kyselina glutamová metabolismus MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- morčata MeSH
- oligopeptidy analýza farmakologie MeSH
- oxid dusnatý metabolismus MeSH
- peptidové fragmenty analýza farmakologie MeSH
- posttranslační úpravy proteinů MeSH
- protein-glutamin:amin-gama-glutamyltransferasa 2 MeSH
- proteiny vázající GTP metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza proteinů MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice * MeSH
- TNF-alfa metabolismus MeSH
- transglutaminasy metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- morčata MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- gliadin MeSH
- glutamin MeSH
- interleukin-10 MeSH
- kyselina glutamová MeSH
- oligopeptidy MeSH
- oxid dusnatý MeSH
- peptidové fragmenty MeSH
- protein-glutamin:amin-gama-glutamyltransferasa 2 MeSH
- proteiny vázající GTP MeSH
- TNF-alfa MeSH
- transglutaminasy MeSH
- VSFQQPQQQYPSSQ MeSH Prohlížeč
Celiac disease, a chronic disorder of the small intestine, is caused by dietary gluten and is characterized by villous atrophy and local inflammation associated with infiltration of B and T lymphocytes and/or macrophages into the intestinal wall. In genetically predisposed individuals, the infiltrating cells are activated by gluten, gliadin and their proteolytic fragments and produce chemokines, cytokines and reactive radicals. The sequence of one of the macrophage-stimulatory gliadin peptic fragment was determined by mass spectrometry (MS) as VSFQQPQQQYPSSQ. The role of tissue transglutaminase (tTG) in innate immunity stimulation was studied by mass spectrometric monitoring of sequence changes in this active peptide. Two sites of glutamine deamidation in this peptide were localized by high-resolution scanning in MS/MS mode in an ion trap. A single deamidation in the parent peptide led to the complete loss of its stimulatory effect on macrophages.
Citace poskytuje Crossref.org
Gliadin peptides activate blood monocytes from patients with celiac disease
