Characterization of the NRAMP1 (SLC11A1) gene in the horse (Equus caballus L.)
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12358853
DOI
10.1046/j.1365-2370.2002.00348.x
PII: 348
Knihovny.cz E-resources
- MeSH
- 5' Untranslated Regions genetics MeSH
- Physical Chromosome Mapping MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Horses MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Cation Transport Proteins genetics MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 5' Untranslated Regions MeSH
- natural resistance-associated macrophage protein 1 MeSH Browser
- Cation Transport Proteins MeSH
The complete coding cDNA sequence of the horse NRAMP1 (SLC11A1) gene was determined (GenBank accession number AF354445). The nucleotide sequence of the horse NRAMP1 gene is similar to sequences of this gene in other species. The gene contains 15 exons whose total length of 1,635 bp corresponds to 544 amino acids constituting the resulting putative protein. Hydrophobicity profile analysis of the deduced horse NRAMP1 gene product showed a nearly identical structure with the mouse NRAMP1 protein. The gene was found to be located on the short arm of ECA 6p12-13 by fluorescence in situ hybridization (FISH) analysis. Five allelic variants of the 5' untranslated region (UTR) were identified at the nucleotide sequence level. PCR-RFLP polymorphisms for NlaIII, TaqI, MspI and AciI were detected. Four out of five alleles could be detected using TaqI and MspI restriction enzymes. Their haplotype frequencies were different in four genetically distinct horse breeds.
References provided by Crossref.org
Positive selection in the SLC11A1 gene in the family Equidae
Polymorphisms in equine immune response genes and their associations with infections
GENBANK
AG354445
