Gender-dependent difference in cell calcium handling in VSMC isolated from SHR: the effect of angiotensin II
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Angiotensin II pharmacology MeSH
- Extracellular Space metabolism MeSH
- Hypertension metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Blood Pressure MeSH
- Rats MeSH
- Sex Characteristics * MeSH
- Rats, Inbred SHR MeSH
- Muscle, Smooth, Vascular drug effects metabolism MeSH
- Thapsigargin pharmacology MeSH
- Calcium metabolism MeSH
- Vasoconstrictor Agents pharmacology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Angiotensin II MeSH
- Enzyme Inhibitors MeSH
- Thapsigargin MeSH
- Calcium MeSH
- Vasoconstrictor Agents MeSH
OBJECTIVE: To investigate gender-dependent difference in the free cytosolic calcium concentration ([Ca2+ ]i ) response to angiotensin II (Ang II) in vascular smooth muscle cells (VSMC) isolated from spontaneously hypertensive rats (SHR). To further evaluate this gender-dependent difference by studying the role of thapsigargin-sensitive intracellular calcium stores and calcium influx in VSMC isolated from male and female SHR. DESIGN AND METHODS: Confluent primary cultures of VSMC isolated from male (n = 14) and female (n = 14) SHR aged 10 weeks were used in this study. [Ca2+ ]i was measured by image analysis of single myocytes loaded with Fura-2. [Ca2+ ]i response of VSMC to Ang II was measured in the presence and absence of extracellular Ca2+, to evaluate the influence of Ca2+ influx. To characterize inositol triphosphate (IP3 )-sensitive sarcoplasmic reticulum calcium stores, thapsigargin-sensitive calcium stores were measured in VSMC isolated from SHR of both genders. RESULTS: VSMC isolated from male SHR were characterized by an augmented [Ca2+ ]i response to angiotensin II in comparison with VSMC isolated from female SHR. Surprisingly, the thapsigargin-stimulated [Ca2+ ]i rise was found to be significantly greater in VSMC isolated from female SHR compared with VSMC isolated from male SHR. On the other hand, the gender-dependent difference in [Ca2+ ]i response to angiotensin II was abolished in the absence of extracellular calcium. CONCLUSIONS: We demonstrated in VSMC isolated from SHR of both genders that a greater [Ca2+ ]i response to angiotensin II in male than female VSMC is dependent on Ca2+ influx.
References provided by Crossref.org
Altered Balance between Vasoconstrictor and Vasodilator Systems in Experimental Hypertension
Research on Experimental Hypertension in Prague (1966-2009)
Hypertension after the Menopause: What Can We Learn from Experimental Studies?
