Influence of polysulfone and hemophan hemodialysis membranes on phagocytes
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
12693710
Knihovny.cz E-zdroje
- MeSH
- antigeny CD11b biosyntéza krev MeSH
- biokompatibilní materiály farmakologie MeSH
- celulosa analogy a deriváty krev farmakologie MeSH
- dialýza ledvin metody MeSH
- fagocyty účinky léků metabolismus MeSH
- HL-60 buňky účinky léků metabolismus MeSH
- L-selektin biosyntéza krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- membrány umělé MeSH
- polymery farmakologie MeSH
- reaktivní formy kyslíku krev metabolismus MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- sulfony krev farmakologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- antigeny CD11b MeSH
- biokompatibilní materiály MeSH
- celulosa MeSH
- Hemophan MeSH Prohlížeč
- L-selektin MeSH
- membrány umělé MeSH
- polymery MeSH
- polysulfone P 1700 MeSH Prohlížeč
- reaktivní formy kyslíku MeSH
- sulfony MeSH
The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro conditions after coincubating them with differentiated HL-60 cells. ROS production was measured using chemiluminometric and flow cytometric methods. Expression of CD11b, CD62L and mitochondrial membrane potential were detected by monoclonal antibodies and by the JC-1 fluorescent probe, respectively. Depressed ROS production was observed in patients already before dialysis. Further decrease in ROS production and an increase in CD11b expression were observed especially in patients after hemophan hemodialysis. Decreased ROS production and increased CD11b expression were observed also after incubation of HL-60 cells with hemophan membranes. Mitochondrial membrane potential dropped only after incubating cells with hemophan membranes proving its more serious adverse effects in comparison with the polysulfone membrane. In conclusion, deleterious effects of hemodialysis on the metabolic activity of phagocytes were proved. Combining chemiluminescent and flow cytometric methods for the detection of ROS production and determining mitochondrial membrane potential can be useful tools for the analysis of material biocompatibility.
