Influence of polysulfone and hemophan hemodialysis membranes on phagocytes
Language English Country Slovakia Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12693710
Knihovny.cz E-resources
- MeSH
- CD11b Antigen biosynthesis blood MeSH
- Biocompatible Materials pharmacology MeSH
- Cellulose analogs & derivatives blood pharmacology MeSH
- Renal Dialysis methods MeSH
- Phagocytes drug effects metabolism MeSH
- HL-60 Cells drug effects metabolism MeSH
- L-Selectin biosynthesis blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Membranes, Artificial MeSH
- Polymers pharmacology MeSH
- Reactive Oxygen Species blood metabolism MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Sulfones blood pharmacology MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- CD11b Antigen MeSH
- Biocompatible Materials MeSH
- Cellulose MeSH
- Hemophan MeSH Browser
- L-Selectin MeSH
- Membranes, Artificial MeSH
- Polymers MeSH
- polysulfone P 1700 MeSH Browser
- Reactive Oxygen Species MeSH
- Sulfones MeSH
The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro conditions after coincubating them with differentiated HL-60 cells. ROS production was measured using chemiluminometric and flow cytometric methods. Expression of CD11b, CD62L and mitochondrial membrane potential were detected by monoclonal antibodies and by the JC-1 fluorescent probe, respectively. Depressed ROS production was observed in patients already before dialysis. Further decrease in ROS production and an increase in CD11b expression were observed especially in patients after hemophan hemodialysis. Decreased ROS production and increased CD11b expression were observed also after incubation of HL-60 cells with hemophan membranes. Mitochondrial membrane potential dropped only after incubating cells with hemophan membranes proving its more serious adverse effects in comparison with the polysulfone membrane. In conclusion, deleterious effects of hemodialysis on the metabolic activity of phagocytes were proved. Combining chemiluminescent and flow cytometric methods for the detection of ROS production and determining mitochondrial membrane potential can be useful tools for the analysis of material biocompatibility.
