AIM: Evaluation of serum levels of 17 cytokines and 5 adhesion molecules in patients with newly diagnosed acute myeloid leukemia (AML) and in healthy subjects using biochip array technology. METHODS: A total of 15 AML patients and 15 healthy subjects (blood donors) were studied. Serum samples were analyzed by biochip based immunoassays on the Evidence Investigator analyzer. This approach allows multi-analytical determination from a single sample. T-tests were used for statistical analysis. RESULTS: In newly diagnosed AML patients, we found significant increase (p < 0.01) in serum VCAM-1, ICAM-1, E-selectin, L-selectin, and significant increase (p < 0.05) in serum IL-6, IL-8. No significant differences were found in the levels of other evaluated cytokines and adhesion molecules. CONCLUSION: Our results indicate that serum levels of specific cytokines and adhesion molecules (VCAM-1, ICAM-1, E-selectin, L-selectin, IL-6, IL-8) are significantly altered in patients with newly diagnosed AML, showing activity of the disease. Whether these alterations could serve as a prognostic marker for AML is not known. Further studies will be needed to define the potential role of these and additional markers in the risk stratification of AML.
- MeSH
- Leukemia, Myeloid, Acute blood genetics pathology MeSH
- Vascular Cell Adhesion Molecule-1 blood MeSH
- Protein Array Analysis methods MeSH
- Cytokines blood MeSH
- Adult MeSH
- E-Selectin blood MeSH
- Interleukin-6 blood MeSH
- Interleukin-8 blood MeSH
- L-Selectin blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Intercellular Adhesion Molecule-1 blood MeSH
- Cell Adhesion Molecules blood MeSH
- Biomarkers, Tumor blood MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Atherogenesis involves the migration of leukocytes into vascular subendothelial space, a process mediated by endothelial and leukocyte cell adhesion molecules. Endothelial molecules are assessed indirectly via serum levels, but leukocyte molecules can be assessed directly. We have therefore hypothesized that leukocyte adhesion molecules are altered to a greater degree in hypercholesterolemia than serum endothelial adhesion molecules. We examined 29 subjects with hypercholesterolemia and 27 controls at baseline and after 12 weeks of atorvastatin treatment (20 mg/day). Expression of leukocyte integrins CD11a, CD11b, CD18, and CD49d and of L-selectin was measured by flow cytometry. Serum ICAM-1, E-selectin and von Willebrand factor were measured by ELISA. Expression of leukocyte adhesion molecules was significantly higher in patients at baseline than in the controls, except for CD11a. Expression significantly decreased after atorvastatin in most adhesion molecules except for CD11b. In contrast, there was no effect of hypercholesterolemia and/or atorvastatin on the serum endothelial molecules. Leukocyte but not endothelial adhesion molecules were influenced by hypercholesterolemia and by lipid lowering treatment. Leukocyte molecules may therefore be a more sensitive marker of atherogenesis than endothelial molecules. Our results support the role of increased leukocyte adhesiveness in atherogenesis.
- MeSH
- Matched-Pair Analysis MeSH
- Anticholesteremic Agents therapeutic use MeSH
- Adult MeSH
- E-Selectin blood drug effects MeSH
- Endothelial Cells metabolism drug effects MeSH
- Hypercholesterolemia drug therapy blood MeSH
- Integrins blood drug effects MeSH
- Heptanoic Acids therapeutic use MeSH
- L-Selectin blood drug effects MeSH
- Leukocytes metabolism drug effects MeSH
- Middle Aged MeSH
- Humans MeSH
- Intercellular Adhesion Molecule-1 blood drug effects MeSH
- Cell Adhesion Molecules blood drug effects MeSH
- Statistics, Nonparametric MeSH
- Pyrroles therapeutic use MeSH
- Reference Values MeSH
- von Willebrand Factor metabolism drug effects MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro conditions after coincubating them with differentiated HL-60 cells. ROS production was measured using chemiluminometric and flow cytometric methods. Expression of CD11b, CD62L and mitochondrial membrane potential were detected by monoclonal antibodies and by the JC-1 fluorescent probe, respectively. Depressed ROS production was observed in patients already before dialysis. Further decrease in ROS production and an increase in CD11b expression were observed especially in patients after hemophan hemodialysis. Decreased ROS production and increased CD11b expression were observed also after incubation of HL-60 cells with hemophan membranes. Mitochondrial membrane potential dropped only after incubating cells with hemophan membranes proving its more serious adverse effects in comparison with the polysulfone membrane. In conclusion, deleterious effects of hemodialysis on the metabolic activity of phagocytes were proved. Combining chemiluminescent and flow cytometric methods for the detection of ROS production and determining mitochondrial membrane potential can be useful tools for the analysis of material biocompatibility.
- MeSH
- CD11b Antigen biosynthesis blood MeSH
- Biocompatible Materials pharmacology MeSH
- Cellulose * analogs & derivatives pharmacology blood MeSH
- Renal Dialysis * methods MeSH
- Phagocytes * metabolism drug effects MeSH
- HL-60 Cells metabolism drug effects MeSH
- L-Selectin biosynthesis blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Membranes, Artificial MeSH
- Polymers * pharmacology MeSH
- Reactive Oxygen Species blood metabolism MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Sulfones pharmacology blood MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- MeSH
- E-Selectin MeSH
- Research Support as Topic MeSH
- Skin injuries MeSH
- L-Selectin MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Cell Adhesion Molecules MeSH
- Mice MeSH
- P-Selectin MeSH
- Wounds and Injuries MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Review MeSH
- Comparative Study MeSH
