Isolation and characterization of Saccharomyces cerevisiae mutants with a different degree of resistance to killer toxins K1 and K2
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12702323
DOI
10.1111/j.1567-1364.2002.tb00070.x
PII: S1567135601000630
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence genetika MeSH
- killer faktory kvasinek MeSH
- membránové proteiny genetika metabolismus MeSH
- mikrobiální testy citlivosti MeSH
- mutace MeSH
- mykotoxiny metabolismus farmakologie MeSH
- receptory buněčného povrchu genetika metabolismus MeSH
- Saccharomyces cerevisiae účinky léků genetika izolace a purifikace metabolismus MeSH
- sféroplasty účinky léků genetika metabolismus MeSH
- testy genetické komplementace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- K1 killer toxin MeSH Prohlížeč
- killer faktory kvasinek MeSH
- membránové proteiny MeSH
- mykotoxiny MeSH
- receptory buněčného povrchu MeSH
Killer toxin K1 of Saccharomyces cerevisiae kills sensitive cells of the same species by disturbing the ion gradient across the plasma membrane after binding to the receptor at cell wall beta-1,6-glucan. Killer protein K2 is assumed to act by a similar mechanism. To identify the putative plasma membrane receptors for both toxins we mutagenized three sensitive S. cerevisiae strains and searched for clones with killer-resistant spheroplasts. The well diffusion assay identified three phenotypically different groups of clones: clones resistant simultaneously to both toxins, clones with lowered sensitivity to only K1 toxin and those with strongly lowered sensitivity to K2 and partially lowered sensitivity to K1 toxin. These phenotypes are controlled by recessive mutations that belong to at least four different complementation groups. This indicates certain differences at the level of interaction of K1 and K2 toxin with sensitive cells.
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