Use of random amplified polymorphic DNA (RAPD) analysis for the identification of Giardia intestinalis subtypes and phylogenetic tree construction
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- druhová specificita MeSH
- fylogeneze * MeSH
- Giardia lamblia klasifikace genetika izolace a purifikace MeSH
- giardiáza klasifikace genetika MeSH
- kočky MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- polymerázová řetězová reakce metody MeSH
- psi MeSH
- savci parazitologie MeSH
- technika náhodné amplifikace polymorfní DNA * MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
A comparison of random amplified polymorphic DNA (RAPD) was used to investigate genetic polymorphisms among 25 isolates of Giardia intestinalis and to assess the utility of RAPD for subtype detection and genealogical analysis. Using data obtained for six human and 19 animal-derived isolates in polymerase chain reactions using 13 different primers, phylogenetic trees were constructed and bootstrap values computed by the program FreeTree. Three major clades were distinguished, corresponding to previously defined genetic assemblages A, B, and E. The purported specificity of assemblage E genotypes for artiodactyl hosts was supported. Assemblages A and B showed wide host spectra, including human and animal hosts. No correlation was found between the genotype of analyzed isolates and the presence or absence of the double-stranded RNA Giardiavirus. The results indicate that RAPD data provide reliable genetic information that can be used for both "fingerprinting" and genealogical purposes.
Citace poskytuje Crossref.org
