Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12948134
DOI
10.1021/ac020788z
Knihovny.cz E-resources
- MeSH
- Adsorption MeSH
- Avidin analysis chemistry genetics MeSH
- Biotin chemistry MeSH
- DNA metabolism MeSH
- Electrochemistry methods MeSH
- Electrodes MeSH
- Plants, Genetically Modified chemistry genetics MeSH
- Nucleic Acid Hybridization methods MeSH
- Zea mays chemistry genetics MeSH
- Streptavidin analysis chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Avidin MeSH
- Biotin MeSH
- DNA MeSH
- Streptavidin MeSH
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
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