Matrix-assisted laser desorption/ionization analysis of lipids and high molecular weight hydrocarbons with lithium 2,5-dihydroxybenzoate matrix
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14515318
DOI
10.1002/rcm.1178
Knihovny.cz E-resources
- MeSH
- Gentisates * MeSH
- Glycerides analysis chemistry MeSH
- Lipids analysis chemistry MeSH
- Plant Leaves chemistry MeSH
- Lithium * MeSH
- Molecular Weight MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Hydrocarbons analysis chemistry MeSH
- Waxes chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2,5-dihydroxybenzoic acid MeSH Browser
- carnauba wax MeSH Browser
- Gentisates * MeSH
- Glycerides MeSH
- Lipids MeSH
- Lithium * MeSH
- Hydrocarbons MeSH
- Waxes MeSH
Lithium 2,5-dihydroxybenzoate (LiDHB) is shown to be a very effective matrix for matrix-assisted laser desorption/ionization (MALDI) analysis of nonpolar long-chain lipids, hydrocarbons and polymers. Under standard desorption and ionization conditions using a conventional nitrogen UV laser (337 nm), hydrocarbons (C(24)-C(40)), diverse lipids (triglycerides, diglycerides, wax esters from leaves) and saturated polymers are effectively lithiated providing [M+Li](+) ions. The formation of lithiated hydrocarbons is not accompanied by an elimination of hydrogen or other fragmentation reactions and, due to the relatively simple isotopic distribution of lithium, seems to be more useable for analysis of hydrocarbon mixtures than the previously used silver cationization agents. The mass calibration can be conveniently performed either externally or internally using poly(ethylene glycol) commercial standards.
References provided by Crossref.org
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