Gamma irradiation results in phosphorylation of p53 at serine-392 in human T-lymphocyte leukaemia cell line MOLT-4
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14680293
Knihovny.cz E-resources
- MeSH
- Apoptosis radiation effects MeSH
- Cyclins metabolism MeSH
- Phosphorylation radiation effects MeSH
- Phosphoserine metabolism MeSH
- Cyclin-Dependent Kinase Inhibitor p21 MeSH
- Leukemia metabolism MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Tumor Suppressor Protein p53 metabolism MeSH
- Flow Cytometry MeSH
- T-Lymphocytes metabolism radiation effects MeSH
- Up-Regulation radiation effects MeSH
- Cell Survival radiation effects MeSH
- Dose-Response Relationship, Radiation MeSH
- Gamma Rays * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CDKN1A protein, human MeSH Browser
- Cyclins MeSH
- Phosphoserine MeSH
- Cyclin-Dependent Kinase Inhibitor p21 MeSH
- Tumor Suppressor Protein p53 MeSH
Exposure of human leukaemia MOLT-4 cells to ionizing irradiation led to apoptosis, which was detected by flow cytometric analysis and degradation of the nuclear lamina. The multiple signalling pathways triggered by either membrane or DNA damage play a critical role in radiation-induced apoptosis. The response to DNA damage is typically associated with the p53 protein accumulation. In this study, we proved that the transcriptionally active p53 variant occurs in the MOLT-4 cells and its abundance alteration is triggered in the gamma-irradiated cell population concomitantly with phosphorylation at both the serine-392 and serine-15 residues. The p21 upregulation followed the p53 phosphorylation process in irradiated MOLT-4 cells.
