IL-10 does not affect oxidative burst and expression of selected surface antigen on human blood phagocytes in vitro
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15046557
Knihovny.cz E-resources
- MeSH
- Lewis X Antigen metabolism MeSH
- CD11b Antigen metabolism MeSH
- Platelet Endothelial Cell Adhesion Molecule-1 metabolism MeSH
- Time Factors MeSH
- Antigens, CD metabolism MeSH
- Phagocytes drug effects metabolism physiology MeSH
- Interleukin-10 administration & dosage pharmacology physiology MeSH
- Interleukin-6 administration & dosage pharmacology MeSH
- Interleukin-8 administration & dosage pharmacology MeSH
- L-Selectin metabolism MeSH
- Leukocytes drug effects metabolism physiology MeSH
- Humans MeSH
- Luminescent Measurements MeSH
- Monocytes drug effects metabolism physiology MeSH
- Neutrophils drug effects metabolism physiology MeSH
- Flow Cytometry MeSH
- Reactive Oxygen Species metabolism MeSH
- Respiratory Burst drug effects physiology MeSH
- Rhodamines chemistry MeSH
- Tetradecanoylphorbol Acetate pharmacology MeSH
- Tumor Necrosis Factor-alpha administration & dosage pharmacology MeSH
- Zymosan pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Lewis X Antigen MeSH
- CD11b Antigen MeSH
- Platelet Endothelial Cell Adhesion Molecule-1 MeSH
- Antigens, CD MeSH
- dihydrorhodamine 123 MeSH Browser
- Interleukin-10 MeSH
- Interleukin-6 MeSH
- Interleukin-8 MeSH
- L-Selectin MeSH
- Reactive Oxygen Species MeSH
- Rhodamines MeSH
- Tetradecanoylphorbol Acetate MeSH
- Tumor Necrosis Factor-alpha MeSH
- Zymosan MeSH
Cytokines play a major role in the control of inflammatory responses, participate in the regulation of blood phagocyte activities and as such are used for immunomodulatory therapy. In the present study, the influence of IL-10 on human blood phagocyte activity in the presence/absence of IL-6, IL-8 and TNF-alpha was tested in vitro. Our research analyzed the effects of cytokines on the production of reactive oxygen species measured by chemiluminescence and flow cytometry, and on the expression of surface molecules (CD11b, CD15, CD62L, CD31) measured by flow cytometry. IL-10 had no inhibitory effect on reactive oxygen species production and the expression of any examined adhesion molecule by resting or stimulated blood phagocytes within 3 h of incubation. Conversely, TNF-alpha, IL-6, and IL-8 increased reactive oxygen species production and the expression of CD11b and CD15 on both neutrophils and monocytes and decreased the expression of CD62L. These priming effects of the tested pro-inflammatory cytokines were not affected by IL-10. The obtained results suggest that IL-10 does not directly control blood phagocyte activation. These results also provide better information about the contribution of IL-6, IL-8 and TNF-alpha to the regulation of blood phagocyte-mediated inflammatory processes.
The potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes
