FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- chromozomy rostlin genetika MeSH
- DNA sondy chemie MeSH
- fluorescenční barviva chemie MeSH
- hybridizace in situ fluorescenční metody MeSH
- in situ značení DNA s primerem metody MeSH
- karbocyaniny chemie MeSH
- lasery MeSH
- metafáze MeSH
- mikrodisekce metody MeSH
- pohlavní chromozomy genetika MeSH
- polymerázová řetězová reakce metody MeSH
- protoplasty cytologie MeSH
- Silene genetika MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyanine dye 3 MeSH Prohlížeč
- DNA sondy MeSH
- fluorescenční barviva MeSH
- karbocyaniny MeSH
We present an improved FISH strategy for differentiating the sex chromosomes of the dioecious model plant, Silene latifolia. Fixed mitotic protoplasts were dropped on a polyethylene naphthalate membrane, the X or Y chromosomes were isolated using nitrogen laser beam microdissection, catapulted by laser pressure, and amplified by DOP-PCR. A modified FAST-FISH protocol based on a short hybridization time combined with a low concentration of probe was used. The success of this approach is demonstrated by the differential labeling of the X and Y chromosomes and it could represent a quick method for comparing organization of plant genomes.
Zobrazit více v PubMed
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