FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia
Language English Country Netherlands Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromosomes, Plant genetics MeSH
- DNA Probes chemistry MeSH
- Fluorescent Dyes chemistry MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Primed In Situ Labeling methods MeSH
- Carbocyanines chemistry MeSH
- Lasers MeSH
- Metaphase MeSH
- Microdissection methods MeSH
- Sex Chromosomes genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Protoplasts cytology MeSH
- Silene genetics MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cyanine dye 3 MeSH Browser
- DNA Probes MeSH
- Fluorescent Dyes MeSH
- Carbocyanines MeSH
We present an improved FISH strategy for differentiating the sex chromosomes of the dioecious model plant, Silene latifolia. Fixed mitotic protoplasts were dropped on a polyethylene naphthalate membrane, the X or Y chromosomes were isolated using nitrogen laser beam microdissection, catapulted by laser pressure, and amplified by DOP-PCR. A modified FAST-FISH protocol based on a short hybridization time combined with a low concentration of probe was used. The success of this approach is demonstrated by the differential labeling of the X and Y chromosomes and it could represent a quick method for comparing organization of plant genomes.
See more in PubMed
Genomics. 1992 Jul;13(3):718-25 PubMed
Plant Cell Physiol. 1999 Jan;40(1):60-8 PubMed
Genome. 1997 Oct;40(5):705-15 PubMed
Hum Mol Genet. 1998;7(10):1619-26 PubMed
Biotechniques. 1999 Aug;27(2):362-7 PubMed
Chromosome Res. 2002;10(7):571-7 PubMed
Biotech Histochem. 1998 May;73(3):150-6 PubMed
Curr Biol. 1996 Feb 1;6(2):149-62 PubMed
Plant Physiol. 2001 Dec;127(4):1418-24 PubMed
Biotechniques. 1998 May;24(5):820-5 PubMed
Sexy ways: approaches to studying plant sex chromosomes
