Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat

. 2004 Nov ; 109 (7) : 1337-45. [epub] 20040910

Jazyk angličtina Země Německo Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid15365624

The analysis of the hexaploid wheat genome (Triticum aestivum L., 2 n=6 x=42) is hampered by its large size (16,974 Mb/1C) and presence of three homoeologous genomes (A, B and D). One of the possible strategies is a targeted approach based on subgenomic libraries of large DNA inserts. In this work, we purified by flow cytometry a total of 10(7) of three wheat D-genome chromosomes: 1D, 4D and 6D. Chromosomal DNA was partially digested with HindIII and used to prepare a specific bacterial artificial chromosome (BAC) library. The library (designated as TA-subD) consists of 87,168 clones, with an average insert size of 85 kb. Among these clones, 53% had inserts larger than 100 kb, only 29% of inserts being shorter than 75 kb. The coverage was estimated to be 3.4-fold, giving a 96.5% probability of identifying a clone corresponding to any sequence on the three chromosomes. Specificity for chromosomes 1D, 4D and 6D was confirmed after screening the library pools with single-locus microsatellite markers. The screening indicated that the library was not biased and gave an estimated coverage of sixfold. This is the second report on BAC library construction from flow-sorted plant chromosomes, which confirms that dissecting of the complex wheat genome and preparation of subgenomic BAC libraries is possible. Their availability should facilitate the analysis of wheat genome structure and evolution, development of cytogenetic maps, construction of local physical maps and map-based cloning of agronomically important genes.

Zobrazit více v PubMed

Theor Appl Genet. 1991 Dec;83(2):209-16 PubMed

Plant Mol Biol. 2002 Mar-Apr;48(5-6):767-90 PubMed

Plant J. 2001 May;26(3):307-16 PubMed

Funct Integr Genomics. 2004 Mar;4(1):12-25 PubMed

Genome. 1999 Dec;42(6):1176-82 PubMed

Genetics. 2004 Oct;168(2):1087-96 PubMed

Genome. 2001 Oct;44(5):883-92 PubMed

Plant J. 2003 Apr;34(2):249-55 PubMed

Chromosome Res. 2004;12(1):77-91 PubMed

Genetics. 2000 Oct;156(2):833-8 PubMed

Plant Biotechnol J. 2004 May;2(3):181-8 PubMed

Cell. 1976 Sep;9(1):91-9 PubMed

Genome. 1997 Feb;40(1):138-42 PubMed

Plant Cell. 2003 May;15(5):1186-97 PubMed

Plant Cell. 2001 Aug;13(8):1735-47 PubMed

Trends Genet. 2002 Nov;18(11):547-50 PubMed

Genetics. 2000 May;155(1):361-7 PubMed

Mol Genet Genomics. 2004 May;271(4):377-86 PubMed

Genome. 2003 Oct;46(5):870-8 PubMed

Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10836-41 PubMed

Genome. 2002 Feb;45(1):189-97 PubMed

Genome. 1993 Feb;36(1):21-31 PubMed

Genome. 2002 Oct;45(5):963-72 PubMed

Genome. 1996 Jun;39(3):543-8 PubMed

Theor Appl Genet. 2002 Jun;104(8):1362-1372 PubMed

Genetics. 1998 Aug;149(4):2007-23 PubMed

Nucleic Acids Res. 1994 Nov 25;22(23):4922-31 PubMed

Nucleic Acids Res. 2000 Dec 15;28(24):E106 PubMed

Theor Appl Genet. 2003 Sep;107(5):931-9 PubMed

Plant J. 2000 Sep;23(5):687-95 PubMed

Proc Natl Acad Sci U S A. 2003 May 13;100(10):6263-8 PubMed

Genome. 2002 Apr;45(2):402-12 PubMed

Theor Appl Genet. 2002 May;104(6-7):1164-1172 PubMed

Funct Integr Genomics. 2003 Mar;3(1-2):56-68 PubMed

Plant J. 2004 Sep;39(6):960-8 PubMed

Chromosoma. 2004 Mar;112(6):288-99 PubMed

Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13436-41 PubMed

Genetics. 2000 Dec;156(4):2033-41 PubMed

Genetics. 2003 May;164(1):311-21 PubMed

Genetics. 2002 May;161(1):345-53 PubMed

Theor Appl Genet. 2003 Mar;106(5):875-82 PubMed

Najít záznam

Citační ukazatele

Možnosti archivace