Expression of beta-galactosidase and beta-xylosidase genes during microspore and pollen development
Language English Country Germany Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Arabidopsis enzymology genetics metabolism MeSH
- beta-Galactosidase chemistry genetics metabolism MeSH
- Phylogeny MeSH
- Spectrometry, Mass, Electrospray Ionization MeSH
- Mass Spectrometry MeSH
- Molecular Weight MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Pollen enzymology genetics growth & development MeSH
- Base Sequence MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Gene Expression Profiling MeSH
- Nicotiana enzymology genetics growth & development MeSH
- Chromatography, High Pressure Liquid MeSH
- Xylosidases chemistry genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- beta-Galactosidase MeSH
- exo-1,4-beta-D-xylosidase MeSH Browser
- Xylosidases MeSH
Tobacco (Nicotiana tabacum L.) microspores at the time of mitosis are characterized by the abundant occurrence of 92- and 98-kDa glycoproteins (GP92 and GP98). GP92 is a soluble protein while GP98 is bound to the insoluble microspore fraction. Both glycoproteins were isolated by affinity chromatography and SDS-PAGE and analysed by MS. Peptide sequences were determined by mu-HPLC/nano-ESI-MS/MS (electrospray ionization tandem MS). GP92 displayed homology to beta-galactosidase (EC 3.2.1.23) and GP98 to beta-xylosidase (EC 3.2.1.37) from Arabidopsis thaliana (L.) Heynh. The activities of the two enzymes in microspore and pollen extracts of tobacco exhibited similar developmental changes to the occurrence of GP92 and GP98, with a maximum around microspore mitosis. These two glycoproteins are the first identified enzymes characteristic of mitotic microspores. Arabidopsis transcriptomic data for five beta-galactosidase and three beta-xylosidase genes abundantly expressed in pollen were verified by reverse transcription-PCR of RNA from different stages of Arabidopsis pollen development and from various parts of the sporophyte. The results showed abundant expression of two genes (At5g20710, At1g31740) homologous to tobacco GP92 in microspores and early pollen, and of three genes (At5g56870, At2g16730 and At4g35010) in maturing pollen. Analysis of beta-xylosidases showed abundant expression of a late pollen-specific gene At3g62710 and low expression of an early gene At5g10560. It is suggested that the early beta-galactosidase and beta-xylosidase genes may participate in cell wall loosening associated with pollen expansion after microspore mitosis and that the products of the late genes may play a role in cell expansion during pollen germination.
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Plant Physiol Biochem. 2004 Feb;42(2):89-96 PubMed
J Biochem. 1982 Dec;92(6):1873-81 PubMed
Plant Cell. 2001 Jul;13(7):1499-510 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Plant Physiol. 2000 Jul;123(3):1173-83 PubMed
J Exp Bot. 2001 Jan;52(354):67-75 PubMed
Plant Mol Biol. 1991 Aug;17(2):295-9 PubMed
Planta. 1986 Sep;168(4):427-32 PubMed
Genome Biol. 2001;2(8):RESEARCH0032 PubMed
Plant Physiol. 2003 Oct;133(2):713-25 PubMed
Plant Physiol. 2003 Jun;132(2):640-52 PubMed
Plant Physiol. 1985 Jan;77(1):225-8 PubMed
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PubMed
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):31-6 PubMed
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D575-7 PubMed
Plant Mol Biol. 1996 May;31(2):213-25 PubMed
Transgenic Res. 1995 Jul;4(4):226-31 PubMed
Proteomics. 2003 May;3(5):738-51 PubMed
Plant Physiol. 1985 Sep;79(1):90-4 PubMed
Development. 1998 Oct;125(19):3789-99 PubMed
Anal Biochem. 1993 Jun;211(2):205-9 PubMed
Plant J. 2003 Feb;33(4):677-90 PubMed
J Exp Bot. 2003 Dec;54(393):2615-22 PubMed
Plant Cell. 2004 Jan;16(1):229-40 PubMed
Plant Mol Biol. 1990 Oct;15(4):605-22 PubMed
Mol Gen Genet. 1992 Sep;234(3):379-89 PubMed
Plant Physiol. 1997 Feb;113(2):377-386 PubMed
Plant Cell. 1993 Oct;5(10):1265-1275 PubMed
