Differential expression of Ixodes ricinus tick genes induced by blood feeding or Borrelia burgdorferi infection
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Borrelia burgdorferi * MeSH
- Gene Library MeSH
- Insect Vectors MeSH
- Genes, Insect genetics MeSH
- Ticks genetics immunology metabolism MeSH
- Ixodes genetics immunology microbiology MeSH
- DNA, Complementary genetics MeSH
- Blood MeSH
- Lyme Disease blood transmission MeSH
- Guinea Pigs MeSH
- Polymerase Chain Reaction MeSH
- Food MeSH
- Eating * MeSH
- Reactive Oxygen Species MeSH
- Gene Expression Regulation physiology MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Animals MeSH
- Check Tag
- Guinea Pigs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Complementary MeSH
- Reactive Oxygen Species MeSH
Ixodes ricinus L. is the principal European vector of Borrelia burgdorferi sensu lato, the causative agent of Lyme borreliosis. Subtractive hybridization was used to isolate tick genes that were induced in whole ticks after blood meals on uninfected and B. burgdorferi-infected guinea pigs. Novel cDNA clones with similarity to cytochrome c oxidase, salivary secreted protein, actin, and a cysteine protease propeptide were induced after a blood meal. Novel cDNA clones with similarity to thioredoxin peroxidases, dolichyl-phosphate beta-glucosyltransferase, glutathione S-transferase, defensin, ML domain-containing protein, and von Willebrand factor were induced after B. burgdorferi infection. Virtual Northern analysis was used to verify that these genes were differentially expressed in ticks after a pathogen-infected blood meal and to detect their tissues of expression. The characterization of genes that are induced after an infected blood meal is essential for gaining an understanding of the molecular mechanisms that underlie vector-pathogen interactions.
References provided by Crossref.org
Deep Sequencing Analysis of the Ixodes ricinus Haemocytome
Interaction of the tick immune system with transmitted pathogens
Functional characterization of two defensin isoforms of the hard tick Ixodes ricinus
IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus