Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16110920
DOI
10.1007/bf02931464
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial analysis MeSH
- Phenotype MeSH
- Lactococcus lactis classification genetics MeSH
- Muramidase genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Repetitive Sequences, Nucleic Acid genetics MeSH
- DNA, Ribosomal analysis MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Random Amplified Polymorphic DNA Technique * MeSH
- Bacterial Typing Techniques MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- AcmA protein, Lactococcus lactis MeSH Browser
- DNA, Bacterial MeSH
- Muramidase MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic identification of Lactococcus lactis strains by RAPD; rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterization.
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