Strains of Lactobacillus paracasei subsp. paracasei (strain ST11BR), L. pentosus (strain ST151BR), L. plantarum (strain ST13BR), and Lactococcus lactis subsp. lactis (strain ST34BR) producing bacteriocin-like peptides were isolated from barley beer produced in the Western, Northern and Eastern provinces of South Africa. The peptides (bacST11BR, bacST151BR, bacST13BR and bacST34BR) lost their activity after treatment with proteinase K, a proteinase, papain, chymotrypsin, trypsin, pepsin and pronase, but not when they were treated with alpha-amylase, suggesting that the peptides are not glycosylated. The peptides inhibited the growth of Lactobacillus casei, L. sakei, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis, but not Enterobacter cloacae, Lactobacillus bulgaricus subsp. delbrueckii, L. plantarum, L. salivarius, Listeria innocua, Staphylococcus aureus, Streptococcus uberis, S. agalactiae, S. caprinus and S. pneumoniae. Peptides bacST11BR and bacST13BR differed from the other 2 peptides by failing to kill Klebsiella pneumoniae and one of the E. coli strains. Peptides were stable after 2 h of incubation at pH 2.0-12.0, and after 90 min at 100 degrees C. When autoclaved (121 degrees C, 20 min), only bacST13BR lost its activity. The bacteriocin-like peptides were produced at a growth temperature of 30 degrees C, but not at 37 degrees C.

Department of Microbiology Stellenbosch University 7600 Stellenbosch South Africa

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J Appl Bacteriol. 1991 Nov;71(5):417-21 PubMed

Appl Microbiol Biotechnol. 1997 Oct;48(4):449-53 PubMed

Meat Sci. 2004 Mar;66(3):703-8 PubMed

Appl Environ Microbiol. 1989 Aug;55(8):1901-6 PubMed

Int J Food Microbiol. 1998 Jul 21;42(3):147-58 PubMed

Int J Food Microbiol. 2002 May 25;75(3):197-212 PubMed

J Appl Bacteriol. 1993 Apr;74(4):406-10 PubMed

Int J Food Microbiol. 1997 Apr 29;36(1):1-29 PubMed

Microbiology (Reading). 1996 Apr;142(4):817-827 PubMed

Biochimie. 1988 Mar;70(3):337-49 PubMed

Appl Microbiol. 1971 May;21(5):943 PubMed

Curr Opin Biotechnol. 2004 Apr;15(2):100-4 PubMed

Lett Appl Microbiol. 1994 Jul;19(1):12-5 PubMed

Appl Microbiol Biotechnol. 1996 Mar;45(1-2):36-40 PubMed

Appl Environ Microbiol. 1999 Dec;65(12):5350-6 PubMed

J Ind Microbiol Biotechnol. 2002 Dec;29(6):303-8 PubMed

Folia Microbiol (Praha). 1998;43(2):151-5 PubMed

Int J Food Microbiol. 2002 Jan 30;72(1-2):155-64 PubMed

Appl Environ Microbiol. 1995 Jan;61(1):175-9 PubMed

Folia Microbiol (Praha). 2003;48(1):56-8 PubMed

Int J Food Microbiol. 2003 Feb 25;81(1):41-52 PubMed

FEMS Microbiol Rev. 1993 Sep;12(1-3):39-85 PubMed

Folia Microbiol (Praha). 2003;48(2):157-61 PubMed

Appl Environ Microbiol. 1999 Mar;65(3):974-81 PubMed

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