Polyomavirus EGFP-pseudocapsids: analysis of model particles for introduction of proteins and peptides into mammalian cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16298367
DOI
10.1016/j.febslet.2005.10.062
PII: S0014-5793(05)01314-1
Knihovny.cz E-resources
- MeSH
- Dendritic Cells metabolism MeSH
- Microscopy, Electron MeSH
- Epithelial Cells metabolism MeSH
- Fibroblasts metabolism MeSH
- Genetic Vectors genetics MeSH
- Interleukin-12 metabolism MeSH
- Humans MeSH
- Mice MeSH
- Peptides genetics MeSH
- Polyomavirus genetics MeSH
- Proteins genetics MeSH
- Transduction, Genetic methods MeSH
- Virion genetics MeSH
- Capsid Proteins genetics MeSH
- Green Fluorescent Proteins genetics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Interleukin-12 MeSH
- Peptides MeSH
- Proteins MeSH
- Capsid Proteins MeSH
- Green Fluorescent Proteins MeSH
A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terminal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion.
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