Polyomavirus EGFP-pseudocapsids: analysis of model particles for introduction of proteins and peptides into mammalian cells
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16298367
DOI
10.1016/j.febslet.2005.10.062
PII: S0014-5793(05)01314-1
Knihovny.cz E-zdroje
- MeSH
- dendritické buňky metabolismus MeSH
- elektronová mikroskopie MeSH
- epitelové buňky metabolismus MeSH
- fibroblasty metabolismus MeSH
- genetické vektory genetika MeSH
- interleukin-12 metabolismus MeSH
- lidé MeSH
- myši MeSH
- peptidy genetika MeSH
- Polyomavirus genetika MeSH
- proteiny genetika MeSH
- transdukce genetická metody MeSH
- virion genetika MeSH
- virové plášťové proteiny genetika MeSH
- zelené fluorescenční proteiny genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- interleukin-12 MeSH
- peptidy MeSH
- proteiny MeSH
- virové plášťové proteiny MeSH
- zelené fluorescenční proteiny MeSH
A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terminal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion.
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