Seasonal dynamics of antibiotic-resistant Enterobacteriaceae in the gastrointestinal tract of domestic sheep
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16408855
DOI
10.1007/bf02931417
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Bacterial * MeSH
- Bacterial Proteins genetics MeSH
- Enterobacteriaceae drug effects genetics isolation & purification MeSH
- Escherichia coli genetics MeSH
- Gastrointestinal Tract microbiology MeSH
- Conjugation, Genetic MeSH
- Microbial Sensitivity Tests MeSH
- Sheep, Domestic microbiology MeSH
- Polymerase Chain Reaction MeSH
- Seasons MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
Considerable variation in counts of antibiotic-resistant enterobacteria in the ovine gastrointestinal tract was observed. The occurrence of ruminal and fecal isolates resistant to ampicillin (Ap), kanamycin (Km) and tetracycline (Tc) culminated in summer months, followed by rapid decline in subsequent months. Using PCR the tem1bla (Apr), aphA1 (Kmr) and tetB (Tcr) genes were found to be predominant. Under in vitro conditions all resistance genes were transferable into laboratory Escherichia coli strain with relatively high frequency (10(-3) transconjugants per recipient).
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