Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants
Language English Country France Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16644231
DOI
10.1016/j.plaphy.2006.02.003
PII: S0981-9428(06)00028-3
Knihovny.cz E-resources
- MeSH
- Acetates pharmacology MeSH
- Brassica napus drug effects enzymology MeSH
- Time Factors MeSH
- Cyclopentanes pharmacology MeSH
- Phospholipase D metabolism MeSH
- Type C Phospholipases metabolism MeSH
- Salicylic Acid pharmacology MeSH
- Plant Leaves enzymology MeSH
- Plant Diseases MeSH
- Oxylipins MeSH
- Plant Growth Regulators pharmacology MeSH
- Plant Proteins metabolism MeSH
- Thiadiazoles pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acetates MeSH
- benzo-1,2,3-thiadiazole MeSH Browser
- Cyclopentanes MeSH
- Phospholipase D MeSH
- Type C Phospholipases MeSH
- Salicylic Acid MeSH
- methyl jasmonate MeSH Browser
- Oxylipins MeSH
- Plant Growth Regulators MeSH
- Plant Proteins MeSH
- Thiadiazoles MeSH
Phospholipid signaling is an important component in eukaryotic signal transduction pathways. In plants, it plays a key role in growth and development as well as in responses to environmental stresses, including pathogen attack. We investigated the involvement of both phospholipase C (PLC, EC 3.1.4.11) and D (PLD, EC 3.1.4.4) in early responses to the treatment of Brassica napus plants with the chemical inducers of systemic acquired resistance (SAR): salicylic acid (SA), benzothiadiazole (BTH), and with the inducer mediating the induced systemic resistance (ISR) pathway, methyl jasmonate (MeJA). Rapid activation (within 0.5-6 h treatment) of the in vitro activity level was found for phosphatidyl inositol 4,5 bisphosphate (PIP2)-specific PLC (PI-PLC) and three enzymatically different forms of PLD: conventional PLDalpha, PIP2-dependent PLD beta/gamma, and oleate-stimulated PLDdelta. The strongest response was found in case of cytosolic PIP2-dependent PLD beta/gamma after BTH treatment. PLDdelta was identified in B. napus leaves and was very rapidly activated after MeJA treatment with the highest degree of activation compared to the other PLD isoforms. Interestingly, an increase in the amount of protein was observed only for PLDgamma and/or delta after ISR induction, but later than the activation occurred. These results show that phospholipases are involved in very early processes leading to systemic responses in plants and that they are most probably initially first activated on post translational level.
References provided by Crossref.org
Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations
Phospholipase D affects translocation of NPR1 to the nucleus in Arabidopsis thaliana
Phosphoglycerolipids are master players in plant hormone signal transduction
