Urinary metabolomic profiling in mice with diet-induced obesity and type 2 diabetes mellitus after treatment with metformin, vildagliptin and their combination
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27164444
DOI
10.1016/j.mce.2016.05.003
PII: S0303-7207(16)30151-4
Knihovny.cz E-zdroje
- Klíčová slova
- Antidiabetic treatment, Mouse, NMR metabolomics, Obesity, Type 2 diabetes mellitus, Urine,
- MeSH
- adamantan analogy a deriváty farmakologie MeSH
- beta-alanin analogy a deriváty metabolismus MeSH
- diabetes mellitus 2. typu farmakoterapie metabolismus moč MeSH
- dieta MeSH
- glukózový toleranční test metody MeSH
- hypoglykemika farmakologie MeSH
- metabolomika metody MeSH
- metformin farmakologie MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- nitrily farmakologie MeSH
- obezita farmakoterapie metabolismus moč MeSH
- pyridony metabolismus MeSH
- pyrrolidiny farmakologie MeSH
- vildagliptin MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 2-hydroxypyridine MeSH Prohlížeč
- 4-pyridone MeSH Prohlížeč
- adamantan MeSH
- beta-alanin MeSH
- hypoglykemika MeSH
- metformin MeSH
- N-carbamoyl-beta-alanine MeSH Prohlížeč
- nitrily MeSH
- pyridony MeSH
- pyrrolidiny MeSH
- vildagliptin MeSH
Metformin, vildagliptin and their combination are widely used for the treatment of diabetes, but little is known about the metabolic responses to these treatments. In the present study, NMR-based metabolomics was applied to detect changes in the urinary metabolomic profile of a mouse model of diet-induced obesity in response to these treatments. Additionally, standard biochemical parameters and the expression of enzymes involved in glucose and fat metabolism were monitored. Significant correlations were observed between several metabolites (e.g., N-carbamoyl-β-alanine, N1-methyl-4-pyridone-3-carboxamide, N1-methyl-2-pyridone-5-carboxamide, glucose, 3-indoxyl sulfate, dimethylglycine and several acylglycines) and the area under the curve of glucose concentrations during the oral glucose tolerance test. The present study is the first to present N-carbamoyl-β-alanine as a potential marker of type 2 diabetes mellitus and consequently to demonstrate the efficacies of the applied antidiabetic interventions. Moreover, the elevated acetate level observed after vildagliptin administration might reflect increased fatty acid oxidation.
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