Urinary metabolomic profiling in mice with diet-induced obesity and type 2 diabetes mellitus after treatment with metformin, vildagliptin and their combination
Language English Country Ireland Media print-electronic
Document type Journal Article
PubMed
27164444
DOI
10.1016/j.mce.2016.05.003
PII: S0303-7207(16)30151-4
Knihovny.cz E-resources
- Keywords
- Antidiabetic treatment, Mouse, NMR metabolomics, Obesity, Type 2 diabetes mellitus, Urine,
- MeSH
- Adamantane analogs & derivatives pharmacology MeSH
- beta-Alanine analogs & derivatives metabolism MeSH
- Diabetes Mellitus, Type 2 drug therapy metabolism urine MeSH
- Diet MeSH
- Glucose Tolerance Test methods MeSH
- Hypoglycemic Agents pharmacology MeSH
- Metabolomics methods MeSH
- Metformin pharmacology MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Nitriles pharmacology MeSH
- Obesity drug therapy metabolism urine MeSH
- Pyridones metabolism MeSH
- Pyrrolidines pharmacology MeSH
- Vildagliptin MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 2-hydroxypyridine MeSH Browser
- 4-pyridone MeSH Browser
- Adamantane MeSH
- beta-Alanine MeSH
- Hypoglycemic Agents MeSH
- Metformin MeSH
- N-carbamoyl-beta-alanine MeSH Browser
- Nitriles MeSH
- Pyridones MeSH
- Pyrrolidines MeSH
- Vildagliptin MeSH
Metformin, vildagliptin and their combination are widely used for the treatment of diabetes, but little is known about the metabolic responses to these treatments. In the present study, NMR-based metabolomics was applied to detect changes in the urinary metabolomic profile of a mouse model of diet-induced obesity in response to these treatments. Additionally, standard biochemical parameters and the expression of enzymes involved in glucose and fat metabolism were monitored. Significant correlations were observed between several metabolites (e.g., N-carbamoyl-β-alanine, N1-methyl-4-pyridone-3-carboxamide, N1-methyl-2-pyridone-5-carboxamide, glucose, 3-indoxyl sulfate, dimethylglycine and several acylglycines) and the area under the curve of glucose concentrations during the oral glucose tolerance test. The present study is the first to present N-carbamoyl-β-alanine as a potential marker of type 2 diabetes mellitus and consequently to demonstrate the efficacies of the applied antidiabetic interventions. Moreover, the elevated acetate level observed after vildagliptin administration might reflect increased fatty acid oxidation.
References provided by Crossref.org
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