Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
16877800
DOI
10.1007/bf03194627
PII: 343
Knihovny.cz E-zdroje
- MeSH
- chov metody MeSH
- DNA fingerprinting metody MeSH
- DNA primery MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- hrách setý genetika MeSH
- polymorfismus genetický * MeSH
- retroelementy genetika MeSH
- shluková analýza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA primery MeSH
- retroelementy MeSH
Fast and efficient DNA fingerprinting of crop cultivars and individuals is frequently used in both theoretical population genetics and in practical breeding. Numerous DNA marker technologies exist and the ratio of speed, cost and accuracy are of importance. Therefore even in species where highly accurate and polymorphic marker systems are available, such as microsatellite SSR (simple sequence repeats), also alternative methods may be of interest. Thanks to their high abundance and ubiquity, temporary mobile retrotransposable elements come into recent focus. Their properties, such as genome wide distribution and well-defined origin of individual insertions by descent, predetermine them for use as molecular markers. In this study, several Ty3-gypsy type retrotransposons have been developed and adopted for the inter-retrotransposon amplified polymorphism (IRAP) method, which is suitable for fast and efficient pea cultivar fingerprinting. The method can easily distinguish even between genetically closely related pea cultivars and provide high polymorphic information content (PIC) in a single PCR analysis.
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Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers
