Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromosomes, Plant genetics MeSH
- DNA, Chloroplast genetics MeSH
- DNA Primers genetics MeSH
- In Situ Hybridization, Fluorescence MeSH
- Models, Genetic MeSH
- Evolution, Molecular MeSH
- Molecular Sequence Data MeSH
- Base Sequence MeSH
- Sequence Homology, Nucleic Acid MeSH
- Silene genetics MeSH
- Chromosomes, Artificial, Bacterial genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Chloroplast MeSH
- DNA Primers MeSH
Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.
References provided by Crossref.org
Evolutionary Variability of W-Linked Repetitive Content in Lacertid Lizards
Impact of Repetitive Elements on the Y Chromosome Formation in Plants
Impact of repetitive DNA on sex chromosome evolution in plants
Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia
Structure and evolution of Apetala3, a sex-linked gene in Silene latifolia
