Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of biting midges (Ceratopogonidae, Diptera)
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17267991
DOI
10.1099/ijs.0.64557-0
Knihovny.cz E-resources
- MeSH
- Ceratopogonidae cytology parasitology ultrastructure MeSH
- Microscopy, Electron MeSH
- Phylogeny MeSH
- Gastrointestinal Tract parasitology MeSH
- Genes, rRNA MeSH
- Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) genetics MeSH
- DNA, Kinetoplast analysis MeSH
- Molecular Sequence Data MeSH
- DNA, Protozoan chemistry genetics MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Protozoan genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- RNA, Ribosomal, 5S genetics MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Nucleic Acid MeSH
- Trypanosomatina classification cytology genetics isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) MeSH
- DNA, Kinetoplast MeSH
- DNA, Protozoan MeSH
- DNA, Ribosomal MeSH
- RNA, Protozoan MeSH
- RNA, Ribosomal, 18S MeSH
- RNA, Ribosomal, 5S MeSH
Three strains of a trypanosomatid protozoan were isolated from the midguts of two naturally infected species of biting midges [Culicoides (Oecacta) festivipennis and Culicoides (Oecacta) truncorum] and characterized by light and electron microscopy and by molecular techniques. Morphological characteristics and sequences of the 18S rRNA, 5S rRNA, spliced leader RNA and glycosomal glyceraldehyde-3-phosphate dehydrogenase genes indicate that the studied flagellates represent a novel phylogenetic lineage within the Trypanosomatidae. Based on phylogenetic analyses, the novel endosymbiont-free, monoxenous trypanosomatid was classified as Sergeia podlipaevi gen. nov., sp. nov. Interestingly, it is closely related to another trypanosomatid species that parasitizes the sand fly Lutzomyia evansi, a blood-sucking dipteran from South America. The type strain of S. podlipaevi sp. nov., ICUL/CZ/2000/CER3, was obtained from Malpighian tubes. Of 2518 females of seven species of biting midges trapped in the Czech Republic, more than 1.5 % were infected by trypanosomatid parasites. An unrelated insect species, Culicoides (Monoculicoides) nubeculosus, was experimentally infected with S. podlipaevi, demonstrating that its host range extends to different subgenera of biting midges.
Biology Centre Institute of Parasitology Czech Academy of Sciences České Budějovice Czech Republic
Department of Parasitology Faculty of Science Charles University Prague Czech Republic
Department of Zoology Faculty of Science Charles University Prague Czech Republic
Faculty of Biology University of South Bohemia České Budějovice Czech Republic
References provided by Crossref.org
Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?
Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids
Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses
The First Non-LRV RNA Virus in Leishmania
Trypanosomatid parasites in Austrian mosquitoes
Biting midges (Ceratopogonidae) as vectors of avian trypanosomes
GENBANK
DQ394362, DQ394363, DQ394364, DQ394365
