Cultivation of human keratinocytes without feeder cells on polymer carriers containing ethoxyethyl methacrylate: in vitro study
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biocompatible Materials chemistry MeSH
- Cell Culture Techniques MeSH
- Keratinocytes cytology physiology MeSH
- Culture Media MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Methacrylates chemistry MeSH
- Polymers chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Culture Media MeSH
- Methacrylates MeSH
- Polymers MeSH
The cell/tissue engineering therapy of extensive or chronic skin wounds is a highly topical task of the contemporary medicine. One of possible therapeutic approaches is grafting of in vitro cultured keratinocytes directly to the wound bed, where the cells colonize the wound, proliferate and improve the re-epithelization process. Because the successful cultivation of keratinocytes needs an application of feeder cells, the exclusion of these cells from the cultivation system is highly required. In this study we show a positive influence of 2-ethoxyethyl methacrylate as a component of cultivation support on growth of keratinocytes without feeder cells. Keratinocytes cultured on these surfaces are able to migrate to the model wound bed in vitro, where they form distinct colonies and have a normal differentiation potential.
See more in PubMed
Cells Tissues Organs. 2002;171(2-3):135-44 PubMed
Int J Dermatol. 2003 Mar;42(3):219-23 PubMed
Wound Repair Regen. 2000 Mar-Apr;8(2):83-96 PubMed
Biotechnol Bioeng. 1998 Sep 20;59(6):679-83 PubMed
Biomaterials. 1999 Nov;20(21):1979-88 PubMed
Biomaterials. 2003 Feb;24(5):863-72 PubMed
J Mater Sci Mater Med. 1997 Oct;8(10):587-90 PubMed
Folia Biol (Praha). 1996;42(3):83-6 PubMed
Biomaterials. 1993 Nov;14(14):1046-50 PubMed
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5665-8 PubMed
J Cell Physiol. 1982 Feb;110(2):219-29 PubMed
Biomaterials. 1998 Jan-Feb;19(1-3):141-6 PubMed
Cancer Microenvironment: What Can We Learn from the Stem Cell Niche
