BACKGROUND AND AIMS: Genome size and chromosome numbers are important cytological characters that significantly influence various organismal traits. However, geographical representation of these data is seriously unbalanced, with tropical and subtropical regions being largely neglected. In the present study, an investigation was made of chromosomal and genome size variation in the majority of Curcuma species from the Indian subcontinent, and an assessment was made of the value of these data for taxonomic purposes. METHODS: Genome size of 161 homogeneously cultivated plant samples classified into 51 taxonomic entities was determined by propidium iodide flow cytometry. Chromosome numbers were counted in actively growing root tips using conventional rapid squash techniques. KEY RESULTS: Six different chromosome counts (2n = 22, 42, 63, >70, 77 and 105) were found, the last two representing new generic records. The 2C-values varied from 1.66 pg in C. vamana to 4.76 pg in C. oligantha, representing a 2.87-fold range. Three groups of taxa with significantly different homoploid genome sizes (Cx-values) and distinct geographical distribution were identified. Five species exhibited intraspecific variation in nuclear DNA content, reaching up to 15.1 % in cultivated C. longa. Chromosome counts and genome sizes of three Curcuma-like species (Hitchenia caulina, Kaempferia scaposa and Paracautleya bhatii) corresponded well with typical hexaploid (2n = 6x = 42) Curcuma spp. CONCLUSIONS: The basic chromosome number in the majority of Indian taxa (belonging to subgenus Curcuma) is x = 7; published counts correspond to 6x, 9x, 11x, 12x and 15x ploidy levels. Only a few species-specific C-values were found, but karyological and/or flow cytometric data may support taxonomic decisions in some species alliances with morphological similarities. Close evolutionary relationships among some cytotypes are suggested based on the similarity in homoploid genome sizes and geographical grouping. A new species combination, Curcuma scaposa (Nimmo) Skornick. & M. Sabu, comb. nov., is proposed.

Department of Botany Charles University Prague Benátská 2 Prague 2 CZ 12801 Czech Republic

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Apavatjrut P, Sirisawad T, Sirirugsa P, Voraurai P, Suwanthada C. Studies on chromosome number of seventeen Thai Curcuma species. Proceedings of 2nd National Conference on Flower and Ornamental Plant; 1996. pp. 86–99.

Ardiyani M. Systematic study of Curcuma L.: turmeric and its allies. Scotland: University of Edinburgh; 2002. PhD thesis.

Beltran IC, Kam YK. Cytotaxonomic studies in the Zingiberaceae. Notes from the Royal Botanic Garden Edinburgh. 1984;41:541–559.

Bennett MD, Leitch IJ. Plant DNA C-values Database (Release 4·0, Oct. 2005) 2005a. http://www.kew.org/genomesize/homepage. (accessed 5 January, 2007).

Bennett MD, Leitch IJ. Nuclear DNA amounts in angiosperms: progress, problems, and prospects. Annals of Botany. 2005b;95:45–90. PubMed PMC

Bharathan G, Lambert G, Galbraith DW. Nuclear DNA content of monocotyledons and related taxa. American Journal of Botany. 1994;81:381–386.

Bhat KG. Curcuma oligantha Trimen (Zingiberaceae) – a new record for India. Indian Journal of Forestry. 1987;10:66–68.

Bisson S, Guillemet S, Hamel J-L. Contribution a l'étude caryo-taxonomique des Scitaminées. Memoires du Museum National d'Histoire Naturelle. Série B. Botanique. 1968;18:59–145.

Chakravorti AK. Multiplication of chromosome numbers in relation to speciation in Zingiberaceae. Science and Culture. 1948;14:137–140.

Chen ZY, Chen SJ. A report on chromosome numbers of Chinese Zingiberaceae. Guihaia. 1984;4:13–18.

Chen ZY, Chen SJ, Huang XX, Huang SF. A report on chromosome numbers on Chinese Zingiberaceae (5) Guihaia. 1988;8:143–147.

Das AB, Rai S, Das P. Karyotype analysis and cytophotometric estimation of nuclear DNA content in some members of the Zingiberaceae. Cytobios. 1999;97:23–33.

Doležel J, Doleželová M, Novák FJ. Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata and M. balbisiana) Biologia Plantarum. 1994;36:351–357.

Doležel J, Greilhuber J, Lucretti S, Meister A, Lysák MA, Nardi L, Obermayer R. Plant genome size estimation by flow cytometry: inter-laboratory comparison. Annals of Botany. 1998;82(Suppl. A):17–26.

Eksomtramage L, Boontum K. Chromosome counts of Zingiberaceae. Songklanakarin Journal of Science and Technology. 1995;17:291–297.

Eksomtramage L., Sirirugsa P, Mayakul S. Chromosome numbers of some Thai Zingiberaceae. Songklanakarin Journal of Science and Technology. 1996a;18:153–159.

Eksomtramage L, Sirirugsa P, Mayakul S. Chromosome counts of Thai Zingiberaceae. In: Wu TL, Wu QG, Chen ZY, editors. Proceedings of the 2nd Symposium on the Family Zingiberaceae; Guangzhou: Zhongshan University Press Press; 1996b. pp. 107–111.

Eksomtramage L, Sirirugsa P, Jivanit P, Maknoi C. Chromosome counts of some zingiberaceous species from Thailand. Songklanakarin Journal of Science and Technology. 2002;24:311–319.

Funakoshi H, Kress JW, Škorničková J, Liu A, Inoue K. Return from the lost: rediscovery of the presumed extinct Leptosolena (Zingiberaceae) in the Philippines and its phylogenetic placement in gingers. Acta Phtyotaxonomica et Geobotanica. 2005;56:41–53.

Grant V. Periodicities in the chromosome numbers of the angiosperms. Botanical Gazette. 1982;143:379–389.

Greilhuber J. Severely distorted Feulgen DNA amounts in Pinus (Coniferophytina) after nonadditive fixations as a result of meristematic self-tanning with vacuole contents. Canadian Journal of Genetics and Cytology. 1986;28:409–415.

Greilhuber J. ‘Self-tanning’ – a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants. Plant Systematics and Evolution. 1988;158:87–96.

Greilhuber J. Intraspecific variation in genome size: a critical reassessment. Annals of Botany. 1998;82(Suppl. A):27–35.

Greilhuber J. Intraspecific variation in genome size in angiosperms: identifying its existence. Annals of Botany. 2005;95:91–98. PubMed PMC

Islam MA. Genetic diversity of the genus Curcuma in Bangladesh and further biotechnological approaches for in vitro regeneration and long-term conservation of C. longa germplasm. Germany: University of Hannover; 2004. PhD thesis.

Joseph R, Joseph T, Joseph J. Karyomorphological studies in the genus Curcuma Linn. Cytologia. 1999;64:313–317.

Kress WJ, Prince LM, Williams KJ. The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. American Journal of Botany. 2002;89:1682–1696. PubMed

Larsen K, Lock JM, Maas H, Maas PJM. Zingiberaceae. In: Kubitzki K., editor. The families and genera of vascular plants. vol. 4. Berlin, Springer-Verlag: 1998. pp. 474–495.

Leitch IJ, Chase MW, Bennett MD. Phylogenetic analysis of DNA C-values provides evidence for a small ancestral genome size in flowering plants. Annals of Botany. 1998;82(Suppl. A):85–94.

Lim SN. Cytogenetics and taxonomy of the genus Globba L. (Zingiberaceae) in Malaya. II. Cytogenetics. Notes Royal Botanic Gardens Edinburgh. 1972a;31:271–284.

Lim SN. Cytogenetics and taxonomy of the genus Globba L. (Zingiberaceae) in Malaya. IV. Distribution in relation to polyploidy. Gardens' Bulletin Singapore. 1972b;26:115–126.

Loureiro J, Rodriguez E, Doležel J, Santos C. Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. Annals of Botany. 2006;98:515–527. PubMed PMC

Lubis I. Phenolic compounds of Curcuma. Annales Bogoriensis. 1968;4:219–225.

Mahelka V, Suda J, Jarolímová V, Trávníček P, Krahulec F. Genome size discriminates between closely related taxa Elytrigia repens and E. intermedia (Poaceae: Triticeae) and their hybrid. Folia Geobotanica. 2005;40:367–384.

Mangaly JK, Sabu M. A taxonomic revision of the South Indian species of Curcuma Linn. (Zingiberaceae) Rheedea. 1993;3:139–171.

Mukherjee I. Chromosome studies of some species of Hedychium. Botanical Magazine Tokyo. 1970;83:237–241.

Murray BG. When does intraspecific C-value variation become taxonomically significant? Annals of Botany. 2005;95:119–125. PubMed PMC

Nambiar MC, Pillai PKT, Sharma YN. Seedling propagation in turmeric (Curcuma aromatica Salisb.) Journal of Plantation Crops. 1982;10:81–85.

Nasir Uddin S. The origin of turmeric: the domestication of an important ginger spice. Scotland: University of Edinburgh; 2000. MSc thesis.

Nayak S, Naik PK, Acharya LK, Pattnaik AK. Detection and evaluation of genetic variation in 17 promising cultivars of turmeric (Curcuma longa L.) using 4C nuclear DNA content and RAPD markers. Cytologia. 2006;71:49–55.

Ngamriabsakul C, Newman MF, Cronk QCB. The phylogeny of tribe Zingibereae (Zingiberaceae) based on ITS (nrDNA) and trnL-F (cpDNA) sequences. Edinburgh Journal of Botany. 2004;57:39–61.

Obermayer R, Greilhuber J. Does genome size in Dasypyrum villosum vary with fruit colour? Heredity. 2005;95:91–95. PubMed

Otto F. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. In: Crissman HA, Darzynkiewicz Z, editors. Methods in cell biology. vol. 33. New York: Academic Press; 1990. pp. 105–110. PubMed

Paisooksantivatana Y, Thepsen O. Phenetic relationship of some Thai Curcuma species (Zingiberaceae) based on morphological, palynological and cytological evidence. Thai Journal of Agricultural Science. 2001;34:47–57.

Poulsen AD. Two new species of Boesenbergia (Zingiberaceae) from Borneo. Nordic Journal of Botany. 1993;13:289–294.

Prana MS. Studies on some Indonesian Curcuma species. England: University of Birmingham; 1977. PhD thesis.

Prana MS, Sastrapradja S, Hawkes JG, Lubis I. A cytological study of some Indonesian Curcuma species. Journal of Root Crops. 1978;4:31–35.

Raghavan RS, Arora CM. Chromosome numbers in Indian medicinal plants. II. Proceedings of Indian Academy of Sciences Series B; 1958. pp. 352–358.

Raghavan TS, Venkatasubban KR. Cytological studies in the family Zingiberaceae with special reference to chromosome number and cyto-taxonomy. Proceedings of Indian Academy of Sciences Series B. 1943;17:118–132.

Ramachandran K. Chromosome numbers in the genus Curcuma Linn. Current Science. 1961;30:194–196.

Ramachandran K. Chromosome numbers in Zingiberaceae. Cytologia. 1969;34:213–221.

Rayburn AL, Auger JA. Nuclear DNA-content variation in the ancient indigenous races of Mexican maize. Acta Botanica Neerlandica. 1990;39:197–202.

Roxburgh W. Descriptions of several of the monandrous plants of India. Asiatick Researches. 1810;11:318–362.

Sabu M. Zingiberaceae and Costaceae of South India. Feroke: Indian Association of Angiosperm Taxonomy; 2006.

Saensouk S, Chantaranothai P. The family Zingiberaceae in Phu Phan National Park. In: Chantaranothai P, Larsen K, Sirirugsa P, Simpson D, editors. Proceedings of the 3rd Symposium on the family Zingiberaceae; Khon Kaen: Applied Taxonomic Research Centre, Khon Kaen University; 2003. pp. 16–25.

Saensouk S, Sumonthip B, Luangpirom A. Chromosome numbers of some Zingiberaceae in Phu Phan National Park. In: Saensouk S, Chantaranothai P, editors. 24th Congress on Science and Technology of Thailand; 19–21 October 1998; Queen Sirikit Botanical Garden; 1998. 2003.

Sarkar AK. Cytological investigation of certain members of Zingiberaceae Lindl. to ascertain their taxonomic affinities. Proceedings of Indian Science Congress. 1990;77:150.

Sato D. Karyotype and systematics of Zingiberales. Japanese Journal of Genetics. 1948;23:44–45.

Sato D. Scientific Papers of the College of General Education. Vol. 10. University of Tokyo; 1960. The karyotype analysis in Zingiberales with special reference to the protokaryotype and stable karyotype; pp. 225–243.

Schumann K. Engler HGA, editor. Zingiberaceae. Das Pflanzenreich. 1904;46:1–458.

Sharma AK, Bhattacharya NK. Cytology of several members of Zingiberaceae. La Cellule. 1959;59:297–346.

Sirirugsa P. The genus Curcuma of Thailand. In: Wu TL, Wu QG, Chen ZY, editors. Proceedings of the 2nd Symposium on the family Zingiberaceae.; Guangzhou: Zhongshan University Press; 1996. pp. 39–46.

Sirisawad T, Sirirugsa P, Suwanthada C, Apavtjrut P. Investigation of chromosome numbers in 20 taxa of Curcuma. In: Chantaranothai P, Larsen K, Sirirugsa P, Simpson D, editors. Proceedings of the 3rd Symposium on the family Zingiberaceae; Khon Kaen: Applied Taxonomic Research Centre, Khon Kaen University; 2003. pp. 54–62.

Škorničková J, Sabu M. The recircumscription of Curcuma L. to include the genus Paracautleya R.M.Sm. Gardens' Bulletin Singapore. 2005a;57:37–46.

Škorničková J, Sabu M. Curcuma roscoeana Wall. (Zingiberaceae) in India. Gardens' Bulletin Singapore. 2005b;57:187–198.

Škorničková J, Sabu M. The identity and distribution of Curcuma zanthorrhiza Roxb. (Zingiberaceae) Gardens' Bulletin Singapore. 2005c;57:199–210.

Škorničková J, Sabu M, Prasanthkumar MG. A new species of Curcuma L. (Zingiberaceae) from Mizoram. Gardens' Bulletin Singapore. 2003a;55:89–95.

Škorničková J, Sabu M, Prasanthkumar MG. Curcuma codonantha (Zingiberaceae) – a new species from the Andaman Islands, India. Gardens' Bulletin Singapore. 2003b;55:219–228.

Škorničková J, Sabu M, Prasanthkumar MG. Curcuma mutabilis (Zingiberaceae): a new species from South India. Gardens' Bulletin Singapore. 2004;56:43–54.

Škorničková J, Rehse T, Sabu M. Other economically important Curcuma species. In: Ravindran PN, Babu KN, Sivaraman K., editors. Turmeric: the genus Curcuma. Boca Raton, FL: CRC Press; 2007. pp. 451–467.

Šmarda P, Bureš P. Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels. Annals of Botany. 2006;98:665–678. PubMed PMC

Smith RM. Synoptic keys to the genera of Zingiberaceae pro parte. Edinburgh: Royal Botanic Garden Edinburgh; 1981.

Stace CA. Cytology and cytogenetics as a fundamental taxonomic resource for the 20th and 21st centuries. Taxon. 2000;49:451–477.

Suguira T. A list of chromosome numbers in angiospermous plants. Botanical Magazine Tokyo. 1931;45:353–355.

Suguira T. Studies on the chromosome numbers in higher plants, with special reference to cytokenesis, I. Cytologia. 1936;7:544–595.

Takano A. Cytological analyses of 19 taxa in Globba (Zingiberaceae) Acta Phytotaxonomica et Geobotanica. 2001;52:65–74.

Takano A, Okada H. Multiple occurrence of triploid formation in Globba (Zingiberaceae) from molecular evidence. Plant Systematics and Evolution. 2002;230:143–159.

Venkatasubban KR. A preliminary survey of chromosome numbers in Scitamineae of Bentham & Hooker. Proceedings of Indian Academy of Sciences Series B; 1946. pp. 281–300.

Walker DJ, Monino I, Correal E. Genome size in Bituminaria bituminosa (L.) C. H. Stirton (Fabaceae) populations: separation of “true” differences from environmental effects on DNA determination. Environmental and Experimental Botany. 2006;55:258–265.

Weerapakdee W, Krasaechai A. Collection and development studies of certain Curcuma spp. Journal of Agriculture. 1997;13:127–136.

Evolutionary importance of the relationship between cytogeography and climate: New insights on creosote bushes from North and South America

Cytotype coexistence in the field cannot be explained by inter-cytotype hybridization alone: linking experiments and computer simulations in the sexual species Pilosella echioides (Asteraceae)

Genome size as a key to evolutionary complex aquatic plants: polyploidy and hybridization in Callitriche (Plantaginaceae)

Continuous morphological variation correlated with genome size indicates frequent introgressive hybridization among Diphasiastrum species (Lycopodiaceae) in Central Europe

Diversity and endemism in deglaciated areas: ploidy, relative genome size and niche differentiation in the Galium pusillum complex (Rubiaceae) in Northern and Central Europe

Bridging global and microregional scales: ploidy distribution in Pilosella echioides (Asteraceae) in central Europe