Photodynamic effect on melanoma cells investigated by atomic force microscopy
Language English Country Slovakia Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18063847
Knihovny.cz E-resources
- MeSH
- Photochemotherapy methods MeSH
- Photosensitizing Agents therapeutic use MeSH
- Humans MeSH
- Melanoma drug therapy ultrastructure MeSH
- Metalloporphyrins therapeutic use MeSH
- Microscopy, Atomic Force methods MeSH
- Cell Line, Tumor MeSH
- Image Enhancement methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosensitizing Agents MeSH
- Metalloporphyrins MeSH
- zinc-tetrakis(p-sulfonatophenyl)porphyrin MeSH Browser
Atomic force microscopy (AFM) is a modern experimental method for imaging of conducting or non-conducting samples. New trends in the application of scanning probe microscopy (SPM) give us the ability to scan live cells directly in their ingenuous surroundings or in air. Our apparatus was replenished with an inverse optical microscope, so we could observe the position of the scanning tip in every individual cell. The aim of the presented study is to picture the cell surface in air. A dry scanner in non-contact or tapping mode was used in the biological application of AFM. In our work the cell line G361 was used as a biological sample. We imaged the cell line before and after induction of a photodynamic effect (PDE) by irradiation of ZnTPPS4-loaded cells with a light dose of 15 J/cm(2). Individual cells before PDE induction had a smooth surface without protrusion on the entire surface. Cells after PDE induction did not have a smooth surface but their surface was rough with protrusion and in some places cleaved.
