LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18160138
DOI
10.1016/j.imlet.2007.11.011
PII: S0165-2478(07)00297-0
Knihovny.cz E-resources
- MeSH
- Alcohol Oxidoreductases MeSH
- Spondylitis, Ankylosing genetics metabolism MeSH
- Autoimmunity genetics MeSH
- Databases, Protein MeSH
- Adult MeSH
- Endopeptidases metabolism MeSH
- Genetic Predisposition to Disease MeSH
- Histones genetics metabolism MeSH
- HLA-B27 Antigen analysis immunology isolation & purification MeSH
- Spectrometry, Mass, Electrospray Ionization * MeSH
- Protein Interaction Domains and Motifs MeSH
- Blood Cells immunology metabolism MeSH
- Humans MeSH
- Peptide Mapping * MeSH
- Peptides analysis immunology isolation & purification MeSH
- HSC70 Heat-Shock Proteins blood genetics immunology MeSH
- GTP-Binding Protein alpha Subunits, Gs blood genetics immunology MeSH
- Sequence Alignment MeSH
- Software MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization * MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Alcohol Oxidoreductases MeSH
- D-2-hydroxyacid dehydrogenase MeSH Browser
- Endopeptidases MeSH
- Histones MeSH
- HLA-B27 Antigen MeSH
- HSPA8 protein, human MeSH Browser
- Peptides MeSH
- HSC70 Heat-Shock Proteins MeSH
- GTP-Binding Protein alpha Subunits, Gs MeSH
Peptides eluted from peripheral blood cells of HLA-B*2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published from in vitro long-term cell cultures available in SYFPEITHI database and splenocytes. It was found that 18 sequences confirmed within 1ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B*2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found.
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