Different solutions used for submucosal injection influenced early healing of gastric endoscopic mucosal resection in a preclinical study in experimental pigs
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
19057952
PubMed Central
PMC2730453
DOI
10.1007/s00464-008-0207-3
Knihovny.cz E-zdroje
- MeSH
- časové faktory MeSH
- deriváty hypromelózy MeSH
- elastická tkáň patologie MeSH
- gastroskopie * MeSH
- glycerol aplikace a dávkování farmakologie terapeutické užití MeSH
- hojení ran účinky léků MeSH
- injekce MeSH
- kolagen analýza MeSH
- lékové roztoky aplikace a dávkování farmakologie terapeutické užití MeSH
- leukocyty účinky léků MeSH
- methylcelulosa aplikace a dávkování analogy a deriváty farmakologie terapeutické užití MeSH
- podskupiny lymfocytů účinky léků MeSH
- preklinické hodnocení léčiv MeSH
- Sus scrofa MeSH
- viskozita MeSH
- žaludeční sliznice účinky léků patologie chirurgie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- deriváty hypromelózy MeSH
- glycerol MeSH
- kolagen MeSH
- lékové roztoky MeSH
- methylcelulosa MeSH
BACKGROUND: We hypothesised that different solutions for submucosal injection may influence early healing of endoscopic mucosal resection (EMR). The aim of this study was to evaluate histological and immunological changes after EMR in experimental pigs. MATERIALS AND METHODS: Two parallel EMRs on the anterior and posterior wall of the gastric body were performed by means of the cap technique in 21 female pigs. A glycerol-based solution (anterior EMR) and hydroxypropyl methylcellulose solution (posterior EMR) were applied for submucosal injection. The animals were sacrificed 7 days later, and tissue sections of all EMRs were stained using combined trichrome. Computer image analysis was used for objective evaluation of elastic and collagen fibres content. Two-colour indirect immunophenotyping of blood and gastric samples were performed using mouse anti-pig monoclonal antibodies. RESULTS: The values of collagen fibre content 7 days after EMR were significantly higher in lesions after the use of solution A in comparison with solution B (2.10 +/- 0.25% versus 1.57 +/- 0.25%, p = 0.009). Concordant results were found in elastic fibres (3.23 +/- 0.49% versus 2.93 +/- 0.61%, p = 0.018). No systemic changes in major leukocyte subpopulations were found. In gastric tissue, lymphocyte subsets exhibited only minor changes. CD4(+) T-lymphocytes were increased in the healing tissue after EMR using solution A (17.08 +/- 9.24% versus 9.76 +/- 7.97%, p = 0.011). Significant increase of SWC3(+) leukocytes was observed after EMR using solution B (47.70 +/- 25.41% versus 18.70 +/- 12.16%, p = 0.001). CONCLUSIONS: The use of glycerol-based solution for submucosal injection was associated with more pronounced histological signs of early healing of EMRs compared with hydroxypropyl methylcellulose.
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Soetikno RM, Gotoda T, Nakanishi Y, Soehendra N (2003) Endoscopic mucosal resection. Gastrointest Endosc 57:567–579 PubMed
None
Tada M, Murata M, Murakami F (1984) Development of strip-off biopsy (in Japanese with English abstract). Gastroenterol Endosc 26:833–839
Inoue H, Takeshita K, Hori H, Muraoka Y, Yoneshima H, Endo M (1993) Endoscopic mucosal resection with a cap-fitted panendoscope for esophagus, stomach, and colon mucosal lesions. Gastrointest Endosc 39:58–62 PubMed
Tanabe S, Koizumi W, Kokutou M, Imaizumi H, Ishii K, Kida M, Yokoyama Y, Ohida M, Saigenji K, Shimao H, Mitomi H (1999) Usefulness of endoscopic aspiration mucosectomy as compared with strip biopsy for the treatment of gastric mucosal cancer. Gastrointest Endosc 50:819–822 PubMed
Shim C-S (2001) Endoscopic mucosal resection: an overview of the value of different techniques. Endoscopy 33:271–275 PubMed
Inoue H, Fukami N, Yoshida T, Kdo SE (2002) Endoscopic mucosal resection for esophageal and gastric cancers. J Gastroenterol Hepatol 17:382–388 PubMed
Norton ID, Wang L, Levine SA, Burgart LJ, Hofmeister EK, Rumalla A, Gostout CJ, Petersen BT (2002) Efficacy of colonic submucosal saline solution injection for the reduction of iatrogenic thermal injury. Gastrointest Endosc 56:95–99 PubMed
Yamamoto H, Koiwai H, Yube T, Isoda N, Sato Y, Sekine Y, Higashizawa T, Utsunomiya K, Ido K, Sugano K (1999) A successful single-step endoscopic resection of a 40 millimeter flat-elevated tumor in the rectum: endoscopic mucosal resection using sodium hyaluronate. Gastrointest Endosc 50:701–704 PubMed
Yamamoto H, Kawata H, Sunada K, Satoh K, Kaneko Y, Ido K, Sugano K (2002) Success rate of curative endoscopic mucosal resection with circumferential mucosal incision assisted by submucosal injection of sodium hyaluronate. Gastrointest Endosc 56:507–512 PubMed
Yamamoto H, Kawata H, Sunada K, Sasaki A, Nakazawa K, Miyata T, Sekine Y, Yano T, Satoh K, Ido K, Sugano K (2003) Successful en-bloc resection of large superficial tumors in the stomach and colon using sodium hyaluronate and small-caliber-tip transparent hood. Endoscopy 35:690–694 PubMed
Fujishiro M, Yahagi N, Kashimura K, Mizushima Y, Oka M, Enomoto S, Kakushima N, Kobayashi K, Hashimoto T, Iguchi M, Shimizu Y, Ichinose M, Omata M (2004) Comparison of various submucosal injection solutions for maintaining mucosal elevation during endoscopic mucosal resection. Endoscopy 36:579–583 PubMed
Uraoka T, Fujii T, Saito Y, Sumiyoshi T, Emura F, Bhandari P, Matsuda T, Fu KI, Saito D (2005) Effectiveness of glycerol as a submucosal injection for EMR. Gastrointest Endosc 61:736–740 PubMed
Fujishiro M, Yahagi N, Nakamura M, Kakushima N, Kodashima S, Ono S, Kobayashi K, Hashimoto T, Yamamichi N, Tateishi A, Shimizu Y, Oka M, Ogura K, Kawabe T, Ichinose M, Omata M (2006) Successful outcomes of a novel endoscopic treatment for GI tumors: endoscopic submucosal dissection with a mixture of high-molecular-weight hyaluronic acid, glycerin, and sugar. Gastrointest Endosc 63:243–249 PubMed
Katsinelos P, Kountouras J, Paroutoglou G, Zavos C, Rizos C, Beltsis A (2006) Endoscopic mucosal resection of large sessile colorectal polyps with submucosal injection of hypertonic 50 percent dextrose-epinephrine solution. Dis Colon Rectum 49:1384–1392 PubMed
Lee S-H, Park J-H, Hyun Park D, Chung IK, Kim HS, Park SH, Kim SJ, Cho HD (2006) Clinical efficacy of EMR with submucosal injection of a fibrinogen mixture: a prospective randomized trial. Gastrointest Endosc 64:691–696 PubMed
Sato T (2006) A novel method of endoscopic mucosal resection assisted by submucosal injection of autologous blood (blood patch EMR). Dis Colon Rectum 49:1636–1641 PubMed
Varadarajulu S, Tamhane A, Slaughter RL (2006) Evaluation of dextrose 50% as a medium for injection-assisted polypectomy. Endoscopy 38:907–912 PubMed
Hurlstone DP, Fu KI, Brown SR, Thomson M, Atkinson R, Tiffin N, Cross SS (2008) EMR using dextrose solution versus sodium hyaluronate for colorectal Paris type I and 0-II lesions: a randomized endoscopist-blinded study. Endoscopy 40:110–114 PubMed
Kararli TT (1995) Comparison of the gastrointestinal anatomy, physiology and biochemistry of humans and commonly used laboratory animals. Biopharm Drug Dispos 16:351–380 PubMed
Fujishiro M, Yahagi N, Kashimura K, Matsuura T, Nakamura M, Kukushima N, Kodashima S, Ono S, Kobayashi K, Hashimoto T, Yamamichi N, Tateishi A, Shimizu Y, Oka M, Ichinose M, Omata M (2005) Tissue damage of different submucosal injection solutions for EMR. Gastrointest Endosc 62:933–942 PubMed
Sinkora J, Rehakova Z, Sinkora M, Cukrowska B, Tlaskalova-Hogenova H, Bianchi AT, De Geus B (1998) Expression of CD2 on porcine B lymphocytes. Immunology 95:443–449 PubMed PMC
Explanatory Report on the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (1986) Council of Europe, Strasbourg
Feitoza AB, Gostout CJ, Burgart LJ, Burkert A, Herman LJ, Rajan E (2003) Hydroxypropyl methylcellulose: a better submucosal fluid cushion for endoscopic mucosal resection. Gastrointest Endosc 57:41–47 PubMed
None
Mitchell RN, Cotran RS (2003) Tissue repair: cell regeneration and fibrosis. In: Kumar V, Cotran RS, Robbins SL (eds) Robbins Basic Pathology, 7th edn. Saunders, Philadelphia, pp 61–78
Oberringer M, Meins C, Bubel M, Pohlemann T (2008) In vitro wounding: effects of hypoxia and transforming growth factor beta(1) on proliferation, migration and myofibroblastic differentiation in an endothelial cell-fibroblast co-culture model. J Mol Histol 39:37–47 PubMed
None PubMed
Fujiwara Y, Arakawa T, Fukuda T, Higuchi K, Kobayashi K, Tarnawski A (1995) Role of extracellular matrix in attachment, migration, and repair of wounded rabbit cultured gastric cells. J Clin Gastroenterol 21(Suppl 1):S125–S130 PubMed
Shahin M, Gillessen A, Pohle T, Weber C, Schuppan D, Herbst H, Domschke W (1997) Gastric ulcer healing in the rat: kinetics and localisation of de novo procollagen synthesis. Gut 41:187–194 PubMed PMC
Gillessen A, Shahin M, Pohle T, Foerster E, Krieg TH, Domschke W (1995) Evidence of de novo collagen synthesis in healing human gastric ulcers. Scand J Gastroenterol 30:515–518 PubMed
Gillessen A, Voss B, Rauterberg J, Domschke W (1993) Distribution of collagen types I, III, and IV in peptic ulcer and normal gastric mucosa in man. Scand J Gastroenterol 28:688–689 PubMed
Nakagawa K, Higuchi K, Arakawa T, Kobayashi K, Kaneda K (2000) Phenotypical and morphological analyses of intraepithelial and lamina propria lymphocytes in normal and regenerating gastric mucosa of rats in comparison with those in intestinal mucosa. Arch Histol Cytol 63:159–167 PubMed
Chamorro S, Revilla C, Alvarez B, Alonso F, Ezquerra A, Dominguez J (2005) Phenotypic and functional heterogeneity of porcine blood monocytes and its relation with maturation. Immunology 114:63–71 PubMed PMC
Kamler JP, Borsatto R, Binmoeller KF (2002) Circumferential endoscopic mucosal resection in the swine esophagus assisted by a cap attachment. Gastrointest Endosc 55:923–928 PubMed
Arepally A, Barnett BP, Montgomery E, Patel TH (2007) Catheter-directed gastric artery chemical embolization for modulation of systemic ghrelin levels in a porcine model: initial experience. Radiology 244:138–143 PubMed
Piasecki C, Wyatt C (1986) Patterns of blood supply to the gastric mucosa. A comparative study revealing an end-artery model. J Anat 149:31–39 PubMed PMC
None PubMed
Seto Y, Nakayama H, Ishigami H, Fujii S, Ueda E (1999) An experimental study of intramural blood supply network of the stomach wall. Hepato-Gastroenterology 46:2673–2676 PubMed
None PubMed
Zamora CS, Reddy VK (1981) Regional blood flow to the stomach and small intestine in swine. Am J Vet Res 42:1531–1533 PubMed
Bacteriocinogeny in experimental pigs treated with indomethacin and Escherichia coli Nissle
Preclinical electrogastrography in experimental pigs
