A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection
Jazyk angličtina Země Německo Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
19757436
DOI
10.1002/elps.200900187
Knihovny.cz E-zdroje
- MeSH
- bolest chemicky indukované farmakoterapie MeSH
- elektrická vodivost MeSH
- elektroforéza kapilární metody MeSH
- glycin analýza metabolismus MeSH
- karagenan farmakologie MeSH
- krysa rodu Rattus MeSH
- neopioidní analgetika farmakologie MeSH
- paracetamol farmakologie MeSH
- potkani Wistar MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- substantia grisea centralis chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- glycin MeSH
- karagenan MeSH
- neopioidní analgetika MeSH
- paracetamol MeSH
CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
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