Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells
Language English Country France Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20106674
DOI
10.1016/j.plaphy.2009.12.011
PII: S0981-9428(10)00006-9
Knihovny.cz E-resources
- MeSH
- Electron Spin Resonance Spectroscopy MeSH
- Photons * MeSH
- Plant Roots chemistry MeSH
- Oxygen chemistry MeSH
- Luminescence * MeSH
- Hydrogen Peroxide pharmacology MeSH
- Raphanus chemistry MeSH
- Reactive Oxygen Species * MeSH
- Reducing Agents pharmacology MeSH
- Free Radical Scavengers MeSH
- Spectrum Analysis MeSH
- Spin Trapping MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Oxygen MeSH
- Hydrogen Peroxide MeSH
- Reactive Oxygen Species * MeSH
- Reducing Agents MeSH
- Free Radical Scavengers MeSH
Biophotons spontaneously emitted from radish root cells were detected using highly sensitive photomultiplier tube. Freshly isolated radish root cells exhibited spontaneous photon emission of about 4 counts s(-1). Addition of hydrogen peroxide to the cells caused significant enhancement in biophoton emission to about 500 counts s(-1). Removal of molecular oxygen using glucose/glucose oxidase system and scavengering of reactive oxygen species by reducing agents such are sodium ascorbate and cysteine completely diminished biophoton emission. Spectral analysis of the hydrogen peroxide-induced biophoton emission indicates that biophotons are emitted mainly in green-red region of the spectra. The data provided by electron paramagnetic resonance spin-trapping technique showed that formation of singlet oxygen observed after addition of H2O2 correlates with enhancement in biophoton emission. These observations provide direct evidence that singlet oxygen is involved in biophoton emission from radish root cells.
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