Characterization of ferritin 2 for the control of tick infestations
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
0757990
Wellcome Trust - United Kingdom
PubMed
20171306
DOI
10.1016/j.vaccine.2010.02.008
PII: S0264-410X(10)00160-X
Knihovny.cz E-resources
- MeSH
- Escherichia coli genetics MeSH
- Ferritins antagonists & inhibitors immunology isolation & purification MeSH
- Insect Proteins antagonists & inhibitors immunology isolation & purification MeSH
- Tick Infestations prevention & control veterinary MeSH
- Ixodes immunology MeSH
- Rabbits parasitology MeSH
- Cattle Diseases parasitology prevention & control MeSH
- Rhipicephalus immunology MeSH
- Cattle MeSH
- Vaccines, Synthetic immunology MeSH
- Animals MeSH
- Check Tag
- Rabbits parasitology MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ferritins MeSH
- Insect Proteins MeSH
- Vaccines, Synthetic MeSH
Ixodes ricinus is one the most abundant tick species in Europe and these ticks transmit pathogens causing human and animal diseases. The cattle ticks, Rhipicephalus (Boophilus) spp., affect cattle production in tropical and subtropical regions of the world. Development of vaccines directed against tick proteins may reduce tick infestations and the transmission of tick-borne pathogens. However, a limiting step in tick vaccine development has been the identification of tick protective antigens. Herein, the tick iron metabolism pathway was targeted in an effort to identify new tick protective antigens. Recombinant I. ricinus (IrFER2) and Rhipicephalus microplus (RmFER2) ferritin 2 proteins were expressed in Escherichia coli and used to immunize rabbits and cattle, respectively. Vaccination with IrFER2 reduced I. ricinus tick numbers, weight and fertility in rabbits with an overall vaccine efficacy (E) of 98%. Control of cattle tick, R. microplus and Rhipicephalus annulatus infestations was obtained in vaccinated cattle with overall E of 64% and 72%, respectively. Notably, the efficacy of the RmFER2 vaccine was similar to that obtained with Bm86 against R. microplus. These collective results demonstrated the feasibility of using ferritin 2 to develop vaccines for the control of tick infestations.
References provided by Crossref.org
Recent Advances in Tick Antigen Discovery and Anti-Tick Vaccine Development
Cattle tick vaccine researchers join forces in CATVAC
Characterization of gut-associated cathepsin D hemoglobinase from tick Ixodes ricinus (IrCD1)
