The use of capillary electrophoresis with contactless conductivity detection for monitoring of glycerol in adipose tissues during a sporting performance
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20564696
DOI
10.1002/elps.201000102
Knihovny.cz E-zdroje
- MeSH
- acetonitrily chemie MeSH
- cvičení fyziologie MeSH
- cyklistika fyziologie MeSH
- elektrická vodivost MeSH
- elektroforéza kapilární metody MeSH
- glycerol analýza metabolismus MeSH
- kalibrace MeSH
- koncentrace vodíkových iontů MeSH
- kyseliny borité chemie MeSH
- lidé MeSH
- lineární modely MeSH
- lipolýza fyziologie MeSH
- mannitol analýza metabolismus MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- sloučeniny lithia chemie MeSH
- tuková tkáň chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- acetonitrile MeSH Prohlížeč
- acetonitrily MeSH
- boric acid MeSH Prohlížeč
- glycerol MeSH
- kyseliny borité MeSH
- lithium hydroxide MeSH Prohlížeč
- mannitol MeSH
- sloučeniny lithia MeSH
A CE procedure employing capacitively coupled contactless conductivity detection has been developed for direct determination of the glycerol and mannitol polyalcohols in biological and pharmacological samples. Both glycerol and mannitol are fully separated from the sample matrix within very short times of 3.0 and 3.9 min, respectively, when using the optimized BGE, 60 mM H3BO3+30 mM LiOH (pH 9.1). The LODs amount to 0.5 microM for glycerol and 0.3 microM for mannitol. The repeatability of the glycerol determination in real biological materials is characterized by the coefficient of variation values, 0.5 and 3.2%, for the migration time and the peak area, respectively. The procedure has been used to monitor the free glycerol concentration in adipose tissue microdialyzates. A physiological study has demonstrated that the lipolysis occurring during a sporting action can be stimulated by local application of adrenaline. The procedure has further been utilized to determine mannitol in a pharmacological preparation.
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