The use of capillary electrophoresis with contactless conductivity detection for monitoring of glycerol in adipose tissues during a sporting performance
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Acetonitriles chemistry MeSH
- Exercise physiology MeSH
- Bicycling physiology MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods MeSH
- Glycerol analysis metabolism MeSH
- Calibration MeSH
- Hydrogen-Ion Concentration MeSH
- Boric Acids chemistry MeSH
- Humans MeSH
- Linear Models MeSH
- Lipolysis physiology MeSH
- Mannitol analysis metabolism MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Lithium Compounds chemistry MeSH
- Adipose Tissue chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- acetonitrile MeSH Browser
- Acetonitriles MeSH
- boric acid MeSH Browser
- Glycerol MeSH
- Boric Acids MeSH
- lithium hydroxide MeSH Browser
- Mannitol MeSH
- Lithium Compounds MeSH
A CE procedure employing capacitively coupled contactless conductivity detection has been developed for direct determination of the glycerol and mannitol polyalcohols in biological and pharmacological samples. Both glycerol and mannitol are fully separated from the sample matrix within very short times of 3.0 and 3.9 min, respectively, when using the optimized BGE, 60 mM H3BO3+30 mM LiOH (pH 9.1). The LODs amount to 0.5 microM for glycerol and 0.3 microM for mannitol. The repeatability of the glycerol determination in real biological materials is characterized by the coefficient of variation values, 0.5 and 3.2%, for the migration time and the peak area, respectively. The procedure has been used to monitor the free glycerol concentration in adipose tissue microdialyzates. A physiological study has demonstrated that the lipolysis occurring during a sporting action can be stimulated by local application of adrenaline. The procedure has further been utilized to determine mannitol in a pharmacological preparation.
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