Effect of mitogen lectin on lymphocyte or brain cortex cell activation
Language English Country Sweden Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20588247
PII: NEL310310A11
Knihovny.cz E-resources
- MeSH
- Lymphocyte Activation drug effects MeSH
- Cell Culture Techniques MeSH
- Potassium pharmacology MeSH
- Phytohemagglutinins pharmacology MeSH
- Mitogens pharmacology MeSH
- Cerebral Cortex drug effects metabolism MeSH
- Mice, Inbred CBA MeSH
- Mice MeSH
- Swine MeSH
- Spleen drug effects metabolism MeSH
- Sodium-Potassium-Exchanging ATPase metabolism MeSH
- T-Lymphocytes metabolism MeSH
- Thymus Gland drug effects metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Potassium MeSH
- Phytohemagglutinins MeSH
- Mitogens MeSH
- phytohemagglutinin L protein, Phaseolus vulgaris MeSH Browser
- Sodium-Potassium-Exchanging ATPase MeSH
OBJECTIVES: We studied a) mitogen lectin (PHA) evoked changes of Na+/K+-ATPase activity in functionally different lymphocytes or brain cortex cells and b) quantitative relationship between PHA- evoked early enzyme activation and late lymphocyte proliferation were analyzed. MATERIALS AND METHODS: We performed biochemical analyses of Pi released from ATP by Na+/K+-ATPase activity. Lymphocyte proliferation was assayed by 3H-thymidine incorporation. RESULTS: We demonstrated PHA stimulated Na+/K+-ATPase activity of mouse spleen lymphocytes or freshly isolated brain cortex cells. Besides this, we estimated high stimulation of Na+/K+-ATPase activity and subsequent late 3H-thymidine incorporation into pig lymphocytes as both PHA dose and K+ ion concentration dependent. CONCLUSIONS: Thus, early PHA dose-dependent stimulation of Na+/K+-ATPase activity is a more general response in different animal species and functionally different cells. We measured both cell type- and PHA-dose dependent enzyme activity stimulation. We can suggest that intensity of early PHA induced Na+/K+-ATPase activation could be in relationship to subsequent elevated level of T lymphocyte proliferation. The Na+/K+-ATPase can be a part of mitogen lectin evoked signal transduction mechanisms.
