Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20626223
DOI
10.33549/physiolres.932010
PII: 932010
Knihovny.cz E-resources
- MeSH
- Action Potentials MeSH
- Artifacts MeSH
- Time Factors MeSH
- Electrophysiologic Techniques, Cardiac * MeSH
- Fluorescence * MeSH
- Fluorescent Dyes MeSH
- Rabbits MeSH
- Perfusion MeSH
- Signal Processing, Computer-Assisted MeSH
- Predictive Value of Tests MeSH
- Reproducibility of Results MeSH
- Heart physiology MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
References provided by Crossref.org
Isolated heart models: cardiovascular system studies and technological advances
