Surface plasmon resonance biosensor for parallelized detection of protein biomarkers in diluted blood plasma
Language English Country Great Britain, England Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
20864329
DOI
10.1016/j.bios.2010.08.063
PII: S0956-5663(10)00582-8
Knihovny.cz E-resources
- MeSH
- Biomarkers blood MeSH
- Antigens, CD blood MeSH
- Chorionic Gonadotropin blood MeSH
- Equipment Design MeSH
- Fetal Proteins MeSH
- Antibodies, Immobilized MeSH
- Immobilized Proteins MeSH
- Blood Proteins analysis MeSH
- Humans MeSH
- Limit of Detection MeSH
- Cell Adhesion Molecules, Neuronal blood MeSH
- Biomarkers, Tumor blood MeSH
- Neoplasms blood diagnosis MeSH
- Oligonucleotides MeSH
- Surface Plasmon Resonance instrumentation methods statistics & numerical data MeSH
- Refractometry MeSH
- Serum Albumin, Bovine MeSH
- Cattle MeSH
- Thionucleotides MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ALCAM protein, human MeSH Browser
- Biomarkers MeSH
- Antigens, CD MeSH
- Chorionic Gonadotropin MeSH
- Fetal Proteins MeSH
- Antibodies, Immobilized MeSH
- Immobilized Proteins MeSH
- Blood Proteins MeSH
- Cell Adhesion Molecules, Neuronal MeSH
- Biomarkers, Tumor MeSH
- Oligonucleotides MeSH
- Serum Albumin, Bovine MeSH
- Thionucleotides MeSH
Surface plasmon resonance (SPR) biosensor for high-throughput screening of protein biomarkers in diluted blood plasma is reported. The biosensor combines a high-resolution SPR imaging sensor and a high-density protein array with low-fouling background. The SPR imaging sensor utilizes polarization contrast and advanced referencing and provides a total of 120 sensing areas (each 200 μm×150 μm). Antibodies are immobilized on the sensing areas via hybridization of antibody-oligonucleotide conjugates to thiolated complementary oligonucleotides microspotted on the sensor surface (DNA-directed immobilization). A low-fouling background is achieved by covalent immobilization of bovine serum albumin to carboxyl-terminated thiols filling the areas among the thiolated oligonucleotides and outside the sensing areas. The biosensor was evaluated for detection of protein biomarkers relevant to cancer diagnostics--human chorionic gonadotropin (hCG) and activated leukocyte cell adhesion molecule (ALCAM) both in buffer and in 10% blood plasma. Limits of detection as low as 45 ng/mL (ALCAM) and 100 ng/mL (hCG) were achieved in blood plasma samples.
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