Cnh1 Na(+) /H(+) antiporter and Ena1 Na(+) -ATPase play different roles in cation homeostasis and cell physiology of Candida glabrata
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- Candida glabrata genetika metabolismus fyziologie MeSH
- delece genu MeSH
- draslík metabolismus MeSH
- fungální proteiny genetika metabolismus MeSH
- homeostáza * MeSH
- kationty metabolismus MeSH
- Na(+)-H(+) antiport genetika metabolismus MeSH
- regulace genové exprese u hub MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- sodík metabolismus MeSH
- substrátová specifita MeSH
- testy genetické komplementace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- CNH1 protein, Candida albicans MeSH Prohlížeč
- draslík MeSH
- fungální proteiny MeSH
- kationty MeSH
- Na(+)-H(+) antiport MeSH
- sodík MeSH
Yeasts tightly regulate their intracellular concentrations of alkali metal cations. In Saccharomyces cerevisiae, the Nha1 Na(+) /H(+) -antiporter and Ena1 Na(+) -ATPase, mediate the efflux of toxic sodium and surplus potassium. We report the characterization of Candida glabrata CgCnh1 and CgEna1 homologues. Their substrate specificity and transport properties were compared upon expression in S. cerevisiae, and their function characterized directly in C. glabrata. The CgCnh1 antiporter and the CgEna1 ATPase transport both potassium and sodium when expressed in S. cerevisiae. CgEna1p fully complements the lack of S. cerevisiae own Na(+) -ATPases but the activity of the CgCnh1 antiporter is lower than that of ScNha1p. Candida glabrata deletion mutants and analyses of their phenotypes revealed that though both transporters have a broad substrate specificity, their function in C. glabrata cells is not the same. Their differing physiological roles are also reflected in their regulation of expression, CgENA1 is highly upregulated by an increased osmotic pressure or sodium concentration, whereas CgCNH1 is expressed constitutively. The Cnh1 antiporter is involved in the regulation of potassium content and the Ena1 ATPase in sodium detoxification of C. glabrata cells. This situation differs from S. cerevisiae, where the Nha1 antiporter and Ena ATPases both participate together in Na(+) detoxification and in the regulation of K(+) homeostasis.
Citace poskytuje Crossref.org
Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness